An integrated widefield imaging and spectroscopy system for contrast-enhanced, image-guided resection of tumors

Aaron M. Mohs, Michael C. Mancini, James M. Provenzale, Corey F. Saba, Karen K. Cornell, Elizabeth W. Howerth, Shuming Nie

Research output: Contribution to journalArticlepeer-review


Tumor recurrence following surgery is a common and unresolved medical problem of great importance since surgery is the most widely used treatment for solid-mass tumors worldwide. A contributing factor to tumor recurrence is the presence of residual tumor remaining at or near the surgical site following surgery. Goal: The primary objective of this study was to develop and evaluate an image-guided surgery system based on a near-infrared, handheld excitation source and spectrograph in combination with a widefield video imaging system. Methods: This system was designed to detect the fluorescence of near-infrared contrast agents and, in particular, indocyanine green (ICG). The imaging system was evaluated for its optical performance and ability to detect the presence of ICG in tumors in an ectopic murine tumor model as well as in spontaneous tumors arising in canines. Results: In both settings, an intravenous ICG infusion provided tumor contrast. In both the murine models and surgical specimens from canines, ICG preferentially accumulated in tumor tissue compared to surrounding normal tissue. The resulting contrast was sufficient to distinguish neoplasia from normal tissue; in the canine surgical specimens, the contrast was sufficient to permit identification of neoplasia on the marginal surface of the specimen. Conclusion: These results demonstrate a unique concept in image-guided surgery by combining local excitation and spectroscopy with widefield imaging. Significance: The ability to readily detect ICG in canines with spontaneous tumors in a clinical setting exemplifies the potential for further clinical translation; the promising results of detecting neoplasia on the marginal specimen surface underscore the clinical utility.

Original languageEnglish (US)
Article number7005462
Pages (from-to)1416-1424
Number of pages9
JournalIEEE Transactions on Biomedical Engineering
Issue number5
StatePublished - May 1 2015
Externally publishedYes


  • ICG
  • Image-guided surgery
  • indocyanine green
  • optical spectroscopy
  • surgical oncology
  • tumors

ASJC Scopus subject areas

  • Biomedical Engineering


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