Abstract
Horseradish has been reported to be frequently sterile and therefore difficult to improve by traditional sexual crosses. In this paper we report a simple method to perform embryo rescues on Murashige and Skoog medium without growth regulators. We then demonstrate the usefulness of the method by making crosses and self-pollinated seeds of several horseradish lines, rescuing the embryos in vitro, and growing the seedlings in soil in a greenhouse. Seedlings obtained in these experiments are being prepared for field plantings. We hope that this simple procedure will speed worldwide efforts to improve horseradish germplasm.
Original language | English (US) |
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Pages (from-to) | 99-105 |
Number of pages | 7 |
Journal | Journal of Vegetable Crop Production |
Volume | 10 |
Issue number | 2 |
DOIs | |
State | Published - Dec 2 2004 |
Keywords
- Armoracia rusticana
- Embryo rescue
- Horseradish
- Tissue culture
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science