An in vitro method for screening for the presence of the pat-2 gene in tomatoes (Lycopersicon esculentum Mill.)

T. E. Young, John A Juvik, J. G. Sullivan, R. M. Skirvin

Research output: Contribution to journalArticlepeer-review

Abstract

Under field conditions, pat-2, the gene which conditions parthenocarpy in tomatoes, is recessive. A simple method has been devised for distinguishing the heterozygote from the two homozygotes using tissue culture. Ovaries of plants segregating for the pat-2 gene were excised and cultured on a medium containing 100 ppm gibberellic acid. After three weeks in culture, three distinct ovary sizes could be seen. It was shown, using F3 progeny tests, that the largest ovaries corresponded to those plants homozygous for the pat-2 gene, the smallest ovaries corresponded to those plants homozygous for the wild type allele, and the intermediate sized ovaries were the heterozygotes. The ability to identify the heterozygote would greatly simplify a backcross breeding program aimed at incorporating the pat-2 gene into commercial cultivars by eliminating the need for an F3 progeny test to determine the genotype of a plant.

Original languageEnglish (US)
Pages (from-to)538-541
Number of pages4
JournalPlant Cell Reports
Volume8
Issue number9
DOIs
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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