Abstract
An improved technique for long-term culture of rice caryopses is necessary for physiological and genetic studies. Panicles of three rice (Oryza sativa) cultivars 'Lemont', 'Gummo-byeu' and 'Hwasung-byeu' were cultured in liquid media with combinations of light versus dark, panicle position, nitrogen level (5-40 mM), and sucrose level (29-351 mM). Grain growth was increased when panicles were positioned horizontally, partially submerged in the media, owing to greater media contact and apoplastic uptake as observed by fluorescent dyes. The optimal media assimilate supply included 175 mM sucrose and 5 mM nitrogen. Grain fill occurred for up to four weeks; grain dry weight reached 80% of that on intact plants, with 50% germination. This technique should allow for future physiological studies with rice or other panicle-bearing species.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 55-60 |
| Number of pages | 6 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 60 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2000 |
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Keywords
- Apoplastic Assimilation
- Culture Medium
- Grain Physiology
- Oryza Sativa
ASJC Scopus subject areas
- Horticulture
Cite this
An improved technique for culture of rice panicles. / Lee, H. J.; Seebauer, J. R.; Below, F. E.
In: Plant Cell, Tissue and Organ Culture, Vol. 60, No. 1, 01.01.2000, p. 55-60.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An improved technique for culture of rice panicles
AU - Lee, H. J.
AU - Seebauer, J. R.
AU - Below, F. E.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - An improved technique for long-term culture of rice caryopses is necessary for physiological and genetic studies. Panicles of three rice (Oryza sativa) cultivars 'Lemont', 'Gummo-byeu' and 'Hwasung-byeu' were cultured in liquid media with combinations of light versus dark, panicle position, nitrogen level (5-40 mM), and sucrose level (29-351 mM). Grain growth was increased when panicles were positioned horizontally, partially submerged in the media, owing to greater media contact and apoplastic uptake as observed by fluorescent dyes. The optimal media assimilate supply included 175 mM sucrose and 5 mM nitrogen. Grain fill occurred for up to four weeks; grain dry weight reached 80% of that on intact plants, with 50% germination. This technique should allow for future physiological studies with rice or other panicle-bearing species.
AB - An improved technique for long-term culture of rice caryopses is necessary for physiological and genetic studies. Panicles of three rice (Oryza sativa) cultivars 'Lemont', 'Gummo-byeu' and 'Hwasung-byeu' were cultured in liquid media with combinations of light versus dark, panicle position, nitrogen level (5-40 mM), and sucrose level (29-351 mM). Grain growth was increased when panicles were positioned horizontally, partially submerged in the media, owing to greater media contact and apoplastic uptake as observed by fluorescent dyes. The optimal media assimilate supply included 175 mM sucrose and 5 mM nitrogen. Grain fill occurred for up to four weeks; grain dry weight reached 80% of that on intact plants, with 50% germination. This technique should allow for future physiological studies with rice or other panicle-bearing species.
KW - Apoplastic Assimilation
KW - Culture Medium
KW - Grain Physiology
KW - Oryza Sativa
UR - http://www.scopus.com/inward/record.url?scp=0033866029&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033866029&partnerID=8YFLogxK
U2 - 10.1023/A:1006477021890
DO - 10.1023/A:1006477021890
M3 - Article
AN - SCOPUS:0033866029
VL - 60
SP - 55
EP - 60
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 1
ER -