TY - JOUR
T1 - An extra copy of the β-glucosidase gene improved the cellobiose fermentation capability of an engineered Saccharomyces cerevisiae strain
AU - Kim, Hyo Jin
AU - Lee, Won Heong
AU - Turner, Timothy Lee
AU - Kwak, Suryang
AU - Jin, Yong Su
N1 - Funding Information:
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2018R1D1A1B07051143) and by a fund from the Energy Biosciences Institute.
Publisher Copyright:
© 2019, King Abdulaziz City for Science and Technology.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - In a previously engineered Saccharomyces cerevisiae recombinant, the cellobiose fermentation rate was significantly lower than the glucose fermentation rate. Thus, we implemented a genome-wide perturbation library to find gene targets for improving the cellobiose fermentation capability of the yeast strain. Unexpectedly, we discovered a transformant that contained an additional β-glucosidase gene (gh1-1), possibly through homologous recombination between the plasmids. The additional β-glucosidase led to the fastest cellobiose fermentation activity among all the transformants evaluated, and the strain demonstrated significantly higher β-glucosidase activity than the control strain, especially during the initial exponential growth phase. The present work revealed the benefit of the extra gh1-1 copy for efficient cellobiose fermentation in the engineered S. cerevisiae strain.
AB - In a previously engineered Saccharomyces cerevisiae recombinant, the cellobiose fermentation rate was significantly lower than the glucose fermentation rate. Thus, we implemented a genome-wide perturbation library to find gene targets for improving the cellobiose fermentation capability of the yeast strain. Unexpectedly, we discovered a transformant that contained an additional β-glucosidase gene (gh1-1), possibly through homologous recombination between the plasmids. The additional β-glucosidase led to the fastest cellobiose fermentation activity among all the transformants evaluated, and the strain demonstrated significantly higher β-glucosidase activity than the control strain, especially during the initial exponential growth phase. The present work revealed the benefit of the extra gh1-1 copy for efficient cellobiose fermentation in the engineered S. cerevisiae strain.
KW - Cellobiose fermentation
KW - Genome-wide overexpression library
KW - Gh1-1
KW - Homologous recombination
KW - Saccharomyces cerevisiae
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U2 - 10.1007/s13205-019-1899-x
DO - 10.1007/s13205-019-1899-x
M3 - Article
C2 - 31588391
AN - SCOPUS:85073264766
SN - 2190-572X
VL - 9
JO - 3 Biotech
JF - 3 Biotech
IS - 10
M1 - 367
ER -