An extra copy of the β-glucosidase gene improved the cellobiose fermentation capability of an engineered Saccharomyces cerevisiae strain

Hyo Jin Kim, Won Heong Lee, Timothy Lee Turner, Suryang Kwak, Yong Su Jin

Research output: Contribution to journalArticlepeer-review

Abstract

In a previously engineered Saccharomyces cerevisiae recombinant, the cellobiose fermentation rate was significantly lower than the glucose fermentation rate. Thus, we implemented a genome-wide perturbation library to find gene targets for improving the cellobiose fermentation capability of the yeast strain. Unexpectedly, we discovered a transformant that contained an additional β-glucosidase gene (gh1-1), possibly through homologous recombination between the plasmids. The additional β-glucosidase led to the fastest cellobiose fermentation activity among all the transformants evaluated, and the strain demonstrated significantly higher β-glucosidase activity than the control strain, especially during the initial exponential growth phase. The present work revealed the benefit of the extra gh1-1 copy for efficient cellobiose fermentation in the engineered S. cerevisiae strain.

Original languageEnglish (US)
Article number367
Journal3 Biotech
Volume9
Issue number10
DOIs
StatePublished - Oct 1 2019

Keywords

  • Cellobiose fermentation
  • Genome-wide overexpression library
  • Gh1-1
  • Homologous recombination
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science (miscellaneous)
  • Agricultural and Biological Sciences (miscellaneous)

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