An ENDOR and HYSCORE investigation of a reaction intermediate in IspG (GcpE) catalysis

Weixue Wang; Ke Wang; Jikun Li; Saritha Nellutla; Tatyana I. Smirnova; Eric Oldfield

doi:10.1021/ja200763a

An ENDOR and HYSCORE investigation of a reaction intermediate in IspG (GcpE) catalysis

Weixue Wang, Ke Wang, Jikun Li, Saritha Nellutla, Tatyana I. Smirnova, Eric Oldfield

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

IspG is a 4Fe-4S protein that carries out an essential reduction step in isoprenoid biosynthesis. Using electron-nuclear double resonance (ENDOR) and hyperfine sublevel correlation (HYSCORE) spectroscopies on labeled samples, we have specifically assigned the hyperfine interactions in a reaction intermediate. These results help clarify the nature of the reaction intermediate, supporting a direct interaction between the unique fourth Fe in the cluster and C2 and O3 of the ligand.

Original language	English (US)
Pages (from-to)	8400-8403
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	133
Issue number	22
DOIs	https://doi.org/10.1021/ja200763a
State	Published - Jun 8 2011

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja200763a

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AU - Li, Jikun

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AU - Smirnova, Tatyana I.

AU - Oldfield, Eric

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An ENDOR and HYSCORE investigation of a reaction intermediate in IspG (GcpE) catalysis

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