An ENDOR and HYSCORE investigation of a reaction intermediate in IspG (GcpE) catalysis

Weixue Wang, Ke Wang, Jikun Li, Saritha Nellutla, Tatyana I. Smirnova, Eric Oldfield

Research output: Contribution to journalArticlepeer-review

Abstract

IspG is a 4Fe-4S protein that carries out an essential reduction step in isoprenoid biosynthesis. Using electron-nuclear double resonance (ENDOR) and hyperfine sublevel correlation (HYSCORE) spectroscopies on labeled samples, we have specifically assigned the hyperfine interactions in a reaction intermediate. These results help clarify the nature of the reaction intermediate, supporting a direct interaction between the unique fourth Fe in the cluster and C2 and O3 of the ligand.

Original languageEnglish (US)
Pages (from-to)8400-8403
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Issue number22
DOIs
StatePublished - Jun 8 2011

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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