@article{6cddb748e28a41bebbc40260092b12ed,
title = "An efficient method and device for transfer of semisolid materials into solid-state NMR spectroscopy rotors",
abstract = "The study of mass-limited biological samples by magic angle spinning (MAS) solid-state NMR spectroscopy critically relies upon the high-yield transfer of material from a biological preparation into the MAS rotor. This issue is particularly important for maintaining biological activity and hydration of semi-solid samples such as membrane proteins in lipid bilayers, pharmaceutical formulations, microcrystalline proteins and protein fibrils. Here we present protocols and designs for rotor-packing devices specifically suited for packing hydrated samples into Pencil-style 1.6 mm, 3.2 mm standard, and 3.2 mm limited speed MAS rotors. The devices are modular and therefore readily adaptable to other rotor and/or ultracentrifugation tube geometries.",
keywords = "Gel phase samples, MAS rotor-packing, Magic angle spinning, Solid-state NMR",
author = "Hisao, {Grant S.} and Harland, {Michael A.} and Brown, {Robert A.} and Berthold, {Deborah A.} and Wilson, {Thomas E.} and Rienstra, {Chad M.}",
note = "Funding Information: This work was supported by grants from the National Institutes of Health ( R01-GM112845 , R01-GM073770 and R21-GM107905 ), the University of Illinois Centennial Scholars Award (to C.M.R.), and the J & M Witt Fellowship (to G.S.H.) The authors would also like to thank Dr. John Stringer and Dr. R. Andrew Byrd for comments on the manuscript, Dr. Kristin M. Nuzzio for discussions in the initial portion of this study, and Prof. Patrick Tranel (University of Illinois at Urbana-Champaign, Department of Crop Sciences) for loaning the Beckman TL-100 ultracentrifuge. The authors would like to dedicate this paper to the memory of our coauthor, Mr. Robert A. Brown, who passed away during the course of this investigation. Publisher Copyright: {\textcopyright} 2016 Elsevier Inc. All rights reserved.",
year = "2016",
month = apr,
day = "1",
doi = "10.1016/j.jmr.2016.01.009",
language = "English (US)",
volume = "265",
pages = "172--176",
journal = "Journal of Magnetic Resonance",
issn = "1090-7807",
publisher = "Academic Press Inc.",
}