An efficient method and device for transfer of semisolid materials into solid-state NMR spectroscopy rotors

Grant S. Hisao, Michael A. Harland, Robert A. Brown, Deborah A. Berthold, Thomas E. Wilson, Chad M. Rienstra

Research output: Contribution to journalArticlepeer-review

Abstract

The study of mass-limited biological samples by magic angle spinning (MAS) solid-state NMR spectroscopy critically relies upon the high-yield transfer of material from a biological preparation into the MAS rotor. This issue is particularly important for maintaining biological activity and hydration of semi-solid samples such as membrane proteins in lipid bilayers, pharmaceutical formulations, microcrystalline proteins and protein fibrils. Here we present protocols and designs for rotor-packing devices specifically suited for packing hydrated samples into Pencil-style 1.6 mm, 3.2 mm standard, and 3.2 mm limited speed MAS rotors. The devices are modular and therefore readily adaptable to other rotor and/or ultracentrifugation tube geometries.

Original languageEnglish (US)
Pages (from-to)172-176
Number of pages5
JournalJournal of Magnetic Resonance
Volume265
DOIs
StatePublished - Apr 1 2016

Keywords

  • Gel phase samples
  • MAS rotor-packing
  • Magic angle spinning
  • Solid-state NMR

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

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