@article{b796246297fe41f6af70c46fb212ff87,
title = "An efficient gene knock-in strategy using 5′-modified double-stranded DNA donors with short homology arms",
abstract = "Here, we report a rapid CRISPR–Cas9-mediated gene knock-in strategy that uses Cas9 ribonucleoprotein and 5′-modified double-stranded DNA donors with 50-base-pair homology arms and achieved unprecedented 65/40% knock-in rates for 0.7/2.5 kilobase inserts, respectively, in human embryonic kidney 293T cells. The identified 5′-end modification led to up to a fivefold increase in gene knock-in rates at various genomic loci in human cancer and stem cells.",
author = "Yi Yu and Yijun Guo and Qiqi Tian and Yuanqing Lan and Hugh Yeh and Meng Zhang and Ipek Tasan and Surbhi Jain and Huimin Zhao",
note = "Funding Information: This work was supported by the US National Institutes of Health (grant nos. 1UM1HG009402 and 1U54DK107965) (H.Z.). We thank B. Pilas and B. Balhan (Flow Cytometry Facility, Biotechnology Center, University of Illinois at Urbana Champaign) for cell sorting experiments. We thank S. Long and K. Sobanska for help with ddPCR experiments. We thank W. Tang, T. Si, J. Lian, C. Field and S. Tsai for helpful suggestions for manuscript writing and experimental designs. Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2020",
month = apr,
doi = "10.1038/s41589-019-0432-1",
language = "English (US)",
volume = "16",
pages = "387--390",
journal = "Nature chemical biology",
issn = "1552-4450",
publisher = "Nature Research",
number = "4",
}