TY - GEN
T1 - An automated microfluidic assay for the detection of cancer biomarkers in serum using photonic crystal enhanced fluorescence
AU - Kwon, Lydia
AU - Race, Caitlin
AU - Foreman, Myles
AU - Cunningham, Brian
N1 - Publisher Copyright:
© 2016 IEEE.
PY - 2016/1/5
Y1 - 2016/1/5
N2 - An automated microfluidic photonic crystal enhanced fluorescence (PCEF) assay platform was recently reported for the multiplexed detection of cytokines spiked into phosphate buffered saline. In this work, we apply the aforementioned platform for cancer biomarker detection in human serum. Detection and control proteins were printed in a microarray format onto the surface of a photonic crystal designed to enhance the signal of fluorophores in the excitation and emission range of Cyanine 5 dye and similar alternatives. Fluorescence-linked immunosorbent assays (FLISAs) were performed, and a dose-response curve was obtained for human serum spiked with anti-E7 antibody, a known biomarker for human papillomavirus (HPV)-associated oropharyngeal cancer (OPC). Further, clinical samples of 20 OPC patients and 20 healthy controls were also tested using the PCEF platform, and the results were compared to those of the gold standard enzyme-linked immunosorbent assay (ELISA). Based on the results of a single trial of forty clinical samples, the sensitivity and specificity of the automated PCEF assay for anti-E7 is 75% and 65%, respectively, compared to 56% and 95% for ELISA.
AB - An automated microfluidic photonic crystal enhanced fluorescence (PCEF) assay platform was recently reported for the multiplexed detection of cytokines spiked into phosphate buffered saline. In this work, we apply the aforementioned platform for cancer biomarker detection in human serum. Detection and control proteins were printed in a microarray format onto the surface of a photonic crystal designed to enhance the signal of fluorophores in the excitation and emission range of Cyanine 5 dye and similar alternatives. Fluorescence-linked immunosorbent assays (FLISAs) were performed, and a dose-response curve was obtained for human serum spiked with anti-E7 antibody, a known biomarker for human papillomavirus (HPV)-associated oropharyngeal cancer (OPC). Further, clinical samples of 20 OPC patients and 20 healthy controls were also tested using the PCEF platform, and the results were compared to those of the gold standard enzyme-linked immunosorbent assay (ELISA). Based on the results of a single trial of forty clinical samples, the sensitivity and specificity of the automated PCEF assay for anti-E7 is 75% and 65%, respectively, compared to 56% and 95% for ELISA.
KW - E7
KW - biosensor
KW - cancer
KW - enhanced fluorescence
KW - human papillomavirus
KW - photonic crystal
KW - serum biomarker
UR - http://www.scopus.com/inward/record.url?scp=85011002736&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85011002736&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2016.7808960
DO - 10.1109/ICSENS.2016.7808960
M3 - Conference contribution
AN - SCOPUS:85011002736
T3 - Proceedings of IEEE Sensors
BT - IEEE Sensors, SENSORS 2016 - Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 15th IEEE Sensors Conference, SENSORS 2016
Y2 - 30 October 2016 through 2 November 2016
ER -