An automated microfluidic assay for the detection of cancer biomarkers in serum using photonic crystal enhanced fluorescence

Lydia Kwon, Caitlin Race, Myles Foreman, Brian Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An automated microfluidic photonic crystal enhanced fluorescence (PCEF) assay platform was recently reported for the multiplexed detection of cytokines spiked into phosphate buffered saline. In this work, we apply the aforementioned platform for cancer biomarker detection in human serum. Detection and control proteins were printed in a microarray format onto the surface of a photonic crystal designed to enhance the signal of fluorophores in the excitation and emission range of Cyanine 5 dye and similar alternatives. Fluorescence-linked immunosorbent assays (FLISAs) were performed, and a dose-response curve was obtained for human serum spiked with anti-E7 antibody, a known biomarker for human papillomavirus (HPV)-associated oropharyngeal cancer (OPC). Further, clinical samples of 20 OPC patients and 20 healthy controls were also tested using the PCEF platform, and the results were compared to those of the gold standard enzyme-linked immunosorbent assay (ELISA). Based on the results of a single trial of forty clinical samples, the sensitivity and specificity of the automated PCEF assay for anti-E7 is 75% and 65%, respectively, compared to 56% and 95% for ELISA.

Original languageEnglish (US)
Title of host publicationIEEE Sensors, SENSORS 2016 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781479982875
DOIs
StatePublished - Jan 5 2017
Event15th IEEE Sensors Conference, SENSORS 2016 - Orlando, United States
Duration: Oct 30 2016Nov 2 2016

Publication series

NameProceedings of IEEE Sensors
ISSN (Print)1930-0395
ISSN (Electronic)2168-9229

Other

Other15th IEEE Sensors Conference, SENSORS 2016
CountryUnited States
CityOrlando
Period10/30/1611/2/16

Keywords

  • E7
  • biosensor
  • cancer
  • enhanced fluorescence
  • human papillomavirus
  • photonic crystal
  • serum biomarker

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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    Kwon, L., Race, C., Foreman, M., & Cunningham, B. (2017). An automated microfluidic assay for the detection of cancer biomarkers in serum using photonic crystal enhanced fluorescence. In IEEE Sensors, SENSORS 2016 - Proceedings [7808960] (Proceedings of IEEE Sensors). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICSENS.2016.7808960