An anaerobic, intrachamber incubator for growth of Methanosarcina spp. on methanol-containing solid media

William W. Metcalf, Jun Kai Zhang, Ralph S. Wolfe

Research output: Contribution to journalArticlepeer-review

Abstract

To simplify the incubation of Methanosarcina spp. on solid agar medium, a two-port, manual, rectangular air lock was modified to serve as an anaerobic incubator. In one operation, it is possible to incubate 153 petri plates, the equivalent of 11 standard anaerobic jars, with plating efficiencies identical to those of traditional protocols.

Original languageEnglish (US)
Pages (from-to)768-770
Number of pages3
JournalApplied and environmental microbiology
Volume64
Issue number2
DOIs
StatePublished - Feb 1998

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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