Amplification and selection of PRRSV-activated VDJ repertoires in pigs secreting distinct neutralizing antiboidies

Nanhua Chen, Benjamin R. Trible, Raymond R.R. Rowland

Research output: Contribution to journalArticlepeer-review

Abstract

Neutralizing antibodies (nAbs) play an important role in protective immunity against porcine reproductive and respiratory syndrome virus (PRRSV) infection. However, the characterization of PRRSV nAb repertoires is rarely investigated. In this study, we developed a swine VDJ amplification method and selection criteria for the characterization of PRRSV-activated VDJ repertoires. According to clonal expansion theory, two separated aliquots of lymph nodes from pigs producing different PRRSV nAbs were utilized to determine the activated B-cell repertoires. Swine VDJ repertoires from a mock-infected pig and PRRSV-infected pigs secreting no detectable nAbs, only homologous nAbs, and broad nAbs were amplified by a single pair of primers that could detect all seven major VDJ genes. The amplicons were cloned and sequenced to generate 385 VDJ sequences. Sequence alignment showed that the diversification of VDJ genes was mainly due to the variation in complementarity determining regions (CDRs), especially CDR3. Based on selection criteria, shared and abundant sequences were identified in two separated aliquots from PRRSV-infected pigs but not from the mock-infected pig, suggesting they were secreted from PRRSV-activated B cells. Thus, the amplification and selection method provide a potential alternative for the characterization of swine VDJ repertoires. However, additional experiments are required to determine whether the shared and abundant VDJ lineages identified in this study are PRRSV-specific or distinct neutralizing-antibodies-associated.

Original languageEnglish (US)
Pages (from-to)53-57
Number of pages5
JournalVeterinary Immunology and Immunopathology
Volume189
DOIs
StatePublished - Jul 2017
Externally publishedYes

Keywords

  • Complementarity determining regions (CDRs)
  • Neutralizing antibodies (nAbs)
  • Porcine reproductive and respiratory syndrome virus (PRRSV)
  • VDJ repertoires

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

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