Amino acid limitation induces down-regulation of WNT5a at transcriptional level

Zuguang Wang, Hong Chen

Research output: Contribution to journalArticle

Abstract

An aberrant WNT signaling contributes to the development and progression of multiple cancers. WNT5a is one of the WNT signaling molecules. This study was designed to test the hypothesis that amino acid deprivation induces changes in the WNT signaling pathway in colon cancer cells. Results showed that targets of the amino acid response pathway, ATF3 and p21, were induced in the human colon cancer cell line SW480 during amino acid limitation. There was a significant decrease in the WNT5a mRNA level following amino acid deprivation. The down-regulation of WNT5a mRNA by amino acid deprivation is not due to mRNA destabilization. There is a reduction of nuclear β-catenin protein level by amino acid limitation. Under amino acid limitation, phosphorylation of ERK1/2 was increased and the blockage of ERK1/2 by the inhibitor U0126 partially restored WNT5a mRNA level. In conclusion, amino acid limitation in colon cancer cells induces phosphorylation of ERK1/2, which then down-regulates WNT5a expression.

Original languageEnglish (US)
Pages (from-to)789-794
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume378
Issue number4
DOIs
StatePublished - Jan 23 2009

Fingerprint

Down-Regulation
Amino Acids
Colonic Neoplasms
Messenger RNA
Phosphorylation
Cells
Catenins
Nuclear Proteins
Cell Line
Molecules
Neoplasms

Keywords

  • AAR
  • Amino acid deprivation
  • ERK
  • MAPK
  • SW480
  • WNT

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Amino acid limitation induces down-regulation of WNT5a at transcriptional level. / Wang, Zuguang; Chen, Hong.

In: Biochemical and Biophysical Research Communications, Vol. 378, No. 4, 23.01.2009, p. 789-794.

Research output: Contribution to journalArticle

@article{6e79e3227abf475f856c243f41993fb8,
title = "Amino acid limitation induces down-regulation of WNT5a at transcriptional level",
abstract = "An aberrant WNT signaling contributes to the development and progression of multiple cancers. WNT5a is one of the WNT signaling molecules. This study was designed to test the hypothesis that amino acid deprivation induces changes in the WNT signaling pathway in colon cancer cells. Results showed that targets of the amino acid response pathway, ATF3 and p21, were induced in the human colon cancer cell line SW480 during amino acid limitation. There was a significant decrease in the WNT5a mRNA level following amino acid deprivation. The down-regulation of WNT5a mRNA by amino acid deprivation is not due to mRNA destabilization. There is a reduction of nuclear β-catenin protein level by amino acid limitation. Under amino acid limitation, phosphorylation of ERK1/2 was increased and the blockage of ERK1/2 by the inhibitor U0126 partially restored WNT5a mRNA level. In conclusion, amino acid limitation in colon cancer cells induces phosphorylation of ERK1/2, which then down-regulates WNT5a expression.",
keywords = "AAR, Amino acid deprivation, ERK, MAPK, SW480, WNT",
author = "Zuguang Wang and Hong Chen",
year = "2009",
month = "1",
day = "23",
doi = "10.1016/j.bbrc.2008.11.124",
language = "English (US)",
volume = "378",
pages = "789--794",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Amino acid limitation induces down-regulation of WNT5a at transcriptional level

AU - Wang, Zuguang

AU - Chen, Hong

PY - 2009/1/23

Y1 - 2009/1/23

N2 - An aberrant WNT signaling contributes to the development and progression of multiple cancers. WNT5a is one of the WNT signaling molecules. This study was designed to test the hypothesis that amino acid deprivation induces changes in the WNT signaling pathway in colon cancer cells. Results showed that targets of the amino acid response pathway, ATF3 and p21, were induced in the human colon cancer cell line SW480 during amino acid limitation. There was a significant decrease in the WNT5a mRNA level following amino acid deprivation. The down-regulation of WNT5a mRNA by amino acid deprivation is not due to mRNA destabilization. There is a reduction of nuclear β-catenin protein level by amino acid limitation. Under amino acid limitation, phosphorylation of ERK1/2 was increased and the blockage of ERK1/2 by the inhibitor U0126 partially restored WNT5a mRNA level. In conclusion, amino acid limitation in colon cancer cells induces phosphorylation of ERK1/2, which then down-regulates WNT5a expression.

AB - An aberrant WNT signaling contributes to the development and progression of multiple cancers. WNT5a is one of the WNT signaling molecules. This study was designed to test the hypothesis that amino acid deprivation induces changes in the WNT signaling pathway in colon cancer cells. Results showed that targets of the amino acid response pathway, ATF3 and p21, were induced in the human colon cancer cell line SW480 during amino acid limitation. There was a significant decrease in the WNT5a mRNA level following amino acid deprivation. The down-regulation of WNT5a mRNA by amino acid deprivation is not due to mRNA destabilization. There is a reduction of nuclear β-catenin protein level by amino acid limitation. Under amino acid limitation, phosphorylation of ERK1/2 was increased and the blockage of ERK1/2 by the inhibitor U0126 partially restored WNT5a mRNA level. In conclusion, amino acid limitation in colon cancer cells induces phosphorylation of ERK1/2, which then down-regulates WNT5a expression.

KW - AAR

KW - Amino acid deprivation

KW - ERK

KW - MAPK

KW - SW480

KW - WNT

UR - http://www.scopus.com/inward/record.url?scp=57849101492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57849101492&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2008.11.124

DO - 10.1016/j.bbrc.2008.11.124

M3 - Article

C2 - 19063862

AN - SCOPUS:57849101492

VL - 378

SP - 789

EP - 794

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -