Ambient ionisation mass spectrometry for lipid profiling and structural analysis of mammalian oocytes, preimplantation embryos and stem cells

Christina R. Ferreira, Alan K. Jarmusch, Valentina Pirro, Clint M. Alfaro, Andres F. González-Serrano, Heiner Niemann, Matthew B. Wheeler, Rathnaweera A C Rabel, Judy E. Hallett, Rebecca Houser, Annemarie Kaufman, R. Graham Cooks

Research output: Contribution to journalReview article

Abstract

Lipids play fundamental roles in mammalian embryo preimplantation development and cell fate. Triacylglycerol accumulates in oocytes and blastomeres as lipid droplets, phospholipids influence membrane functional properties, and essential fatty acid metabolism is important for maintaining the stemness of cells cultured in vitro. The growing impact that lipids have in the field of developmental biology makes analytical approaches to analyse structural information of great interest. This paper describes the concept and presents the results of lipid profiling by mass spectrometry (MS) of oocytes and preimplantation embryos, with special focus on ambient ionisation. Based on our previous experience with oocytes and embryos, we aim to convey that ambient MS is also valuable for stem cell differentiation analysis. Ambient ionisation MS allows the detection of a wide range of lipid classes (e.g. free fatty acids, cholesterol esters, phospholipids) in single oocytes, embryos and cell pellets, which are informative of in vitro culture impact, developmental and differentiation stages. Background on MS principles, the importance of underused MS scan modes for structural analysis of lipids, and statistical approaches used for data analysis are covered. We envisage that MS alone or in combination with other techniques will have a profound impact on the understanding of lipid metabolism, particularly in early embryo development and cell differentiation research.

Original languageEnglish (US)
Pages (from-to)621-637
Number of pages17
JournalReproduction, Fertility and Development
Volume27
Issue number4
DOIs
StatePublished - Jan 1 2015

Fingerprint

Blastocyst
ionization
Oocytes
stem cells
Mass Spectrometry
embryo (animal)
oocytes
Stem Cells
mass spectrometry
Lipids
lipids
cell differentiation
Embryonic Development
Cell Differentiation
Phospholipids
phospholipids
embryogenesis
Embryonic Structures
Mammalian Embryo
Developmental Biology

Keywords

  • desorption electrospray ionization
  • multivariate statistics

ASJC Scopus subject areas

  • Biotechnology
  • Reproductive Medicine
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology

Cite this

Ferreira, C. R., Jarmusch, A. K., Pirro, V., Alfaro, C. M., González-Serrano, A. F., Niemann, H., ... Cooks, R. G. (2015). Ambient ionisation mass spectrometry for lipid profiling and structural analysis of mammalian oocytes, preimplantation embryos and stem cells. Reproduction, Fertility and Development, 27(4), 621-637. https://doi.org/10.1071/RD14310

Ambient ionisation mass spectrometry for lipid profiling and structural analysis of mammalian oocytes, preimplantation embryos and stem cells. / Ferreira, Christina R.; Jarmusch, Alan K.; Pirro, Valentina; Alfaro, Clint M.; González-Serrano, Andres F.; Niemann, Heiner; Wheeler, Matthew B.; Rabel, Rathnaweera A C; Hallett, Judy E.; Houser, Rebecca; Kaufman, Annemarie; Cooks, R. Graham.

In: Reproduction, Fertility and Development, Vol. 27, No. 4, 01.01.2015, p. 621-637.

Research output: Contribution to journalReview article

Ferreira, CR, Jarmusch, AK, Pirro, V, Alfaro, CM, González-Serrano, AF, Niemann, H, Wheeler, MB, Rabel, RAC, Hallett, JE, Houser, R, Kaufman, A & Cooks, RG 2015, 'Ambient ionisation mass spectrometry for lipid profiling and structural analysis of mammalian oocytes, preimplantation embryos and stem cells', Reproduction, Fertility and Development, vol. 27, no. 4, pp. 621-637. https://doi.org/10.1071/RD14310
Ferreira, Christina R. ; Jarmusch, Alan K. ; Pirro, Valentina ; Alfaro, Clint M. ; González-Serrano, Andres F. ; Niemann, Heiner ; Wheeler, Matthew B. ; Rabel, Rathnaweera A C ; Hallett, Judy E. ; Houser, Rebecca ; Kaufman, Annemarie ; Cooks, R. Graham. / Ambient ionisation mass spectrometry for lipid profiling and structural analysis of mammalian oocytes, preimplantation embryos and stem cells. In: Reproduction, Fertility and Development. 2015 ; Vol. 27, No. 4. pp. 621-637.
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