TY - JOUR
T1 - Aluminum inhibits the H+-ATpase activity by permanently altering the plasma membrane surface potentials in squash roots
AU - Sung Ju Ahn, Ju Ahn
AU - Sivaguru, M.
AU - Osawa, H.
AU - Gap Chae Chung, Chae Chung
AU - Matsumoto, H.
PY - 2001
Y1 - 2001
N2 - Although aluminum (AL) toxicity has been widely studied in monocotyledonous crop plants, the mechanism of Al impact on economically important dicotyledonous plants is poorly understood. Here, we report the spatial pattern of Al-induced root growth inhibition, which is closely associated with inhibition of H+-ATPase activity coupled with decreased surface negativity of plasma membrane (PM) vesicles isolated from apical 5-mm root segments of squash (Cucurbita pepo L. cv Tetsukabuto) plants. High-sensitivity growth measurements indicated that the central elongation zone, located 2 to 4 mm from the tip, was preferentially inhibited where high Al accumulation was found. The highest positive shifts (depolarization) in zeta potential of the isolated PM vesicles from 0- to 5-mm regions of Al-treated roots were corresponded to pronounced inhibition of H+-ATPase activity. The depolarization of PM vesicles isolated from Al-treated roots in response to added Al in vitro was less than that of control roots, suggesting, particularly in the first 5-mm root apex, a tight Al binding to PM target sites or irreversible alteration of PM properties upon Al treatment to intact plants. In line with these data, immunolocalization of H+-ATPase revealed decreases in tissue-specific H+-ATPase in the epidermal and cortex cells (2-3 mm from tip) following Al treatments. Our report provides the first circumstantial evidence for a zone-specific depolarization of PM surface potential coupled with inhibition of H+-ATPase activity. These effects may indicate a direct Al interaction with H+-ATPase from the cytoplasmic side of the PM.
AB - Although aluminum (AL) toxicity has been widely studied in monocotyledonous crop plants, the mechanism of Al impact on economically important dicotyledonous plants is poorly understood. Here, we report the spatial pattern of Al-induced root growth inhibition, which is closely associated with inhibition of H+-ATPase activity coupled with decreased surface negativity of plasma membrane (PM) vesicles isolated from apical 5-mm root segments of squash (Cucurbita pepo L. cv Tetsukabuto) plants. High-sensitivity growth measurements indicated that the central elongation zone, located 2 to 4 mm from the tip, was preferentially inhibited where high Al accumulation was found. The highest positive shifts (depolarization) in zeta potential of the isolated PM vesicles from 0- to 5-mm regions of Al-treated roots were corresponded to pronounced inhibition of H+-ATPase activity. The depolarization of PM vesicles isolated from Al-treated roots in response to added Al in vitro was less than that of control roots, suggesting, particularly in the first 5-mm root apex, a tight Al binding to PM target sites or irreversible alteration of PM properties upon Al treatment to intact plants. In line with these data, immunolocalization of H+-ATPase revealed decreases in tissue-specific H+-ATPase in the epidermal and cortex cells (2-3 mm from tip) following Al treatments. Our report provides the first circumstantial evidence for a zone-specific depolarization of PM surface potential coupled with inhibition of H+-ATPase activity. These effects may indicate a direct Al interaction with H+-ATPase from the cytoplasmic side of the PM.
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U2 - 10.1104/pp.126.4.1381
DO - 10.1104/pp.126.4.1381
M3 - Article
C2 - 11500538
AN - SCOPUS:0034863912
SN - 0032-0889
VL - 126
SP - 1381
EP - 1390
JO - Plant physiology
JF - Plant physiology
IS - 4
ER -