Altered Purkinje cell responses and calmodulin expression in the spontaneously ataxic mouse, Pogo

Kwan Young Lee, Jin Seong Kim, Se Hoon Kim, Hyung Seo Park, Young Gil Jeong, Nam Seob Lee, Dong Kwan Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Ataxia is often associated with altered cerebellar motor control, a process in which Purkinje cells (PCs) play a principal role. Pogo mice display severe motor deficits characterized by an ataxic gait accompanying hindlimb hyperextension. Here, using whole-cell patch-clamp recordings, we show that parallel fiber (PF)-excitatory post-synaptic currents (PF-EPSCs) are reduced, paired-pulse facilitation (PPF) is increased and PF-PC long-term depression (LTD) is impaired in Pogo mice; in contrast, climbing-fiber EPSCs are preserved. In control mice, treatment with the calmodulin antagonist calmidazolium (5μm) impaired PPF and LTD. Notably, cerebellar calmodulin expression was significantly reduced in Pogo mice compared with control mice. Control PCs predominantly exhibited a tonic firing pattern, whereas the firing pattern in Pogo PCs was mainly a complex burst type. These results implicate alterations in PC responses and calmodulin content in the abnormal cerebellar function of Pogo mice.

Original languageEnglish (US)
Pages (from-to)1493-1503
Number of pages11
JournalEuropean Journal of Neuroscience
Volume33
Issue number8
DOIs
StatePublished - Apr 1 2011
Externally publishedYes

Keywords

  • Calmodulin
  • Cerebellum
  • Electrophysiology
  • Firing pattern
  • Long-term depression

ASJC Scopus subject areas

  • Neuroscience(all)

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