Alterations in blood plasma and milk fatty acid profiles of lactating Holstein cows in response to ruminal infusion of a conjugated linoleic acid mixture

Juan J Loor, Joseph H. Herbein

Research output: Contribution to journalArticle

Abstract

The production of intermediates during hydrogenation of conjugated linoleic acid (CLA) isomers was determined by infusing a CLA mixture (41% cis9,trans 11-18:2 and 44% trans 10,cis12-18:2) into the rumen of lactating cows. Four Holstein cows fed a basal diet were infused for 48 h with doses of 0, 45, 90, or 180 g CLA·d-1 into the rumen. Treatments were randomly assigned in a 4 × 4 Latin square with 4-d experimental periods, and a 7-d transition between periods. Milk samples were obtained at -12 and 0 h before infusion, and at 12 h intervals from 0 to 96 h after infusion. Milk yield and DMI were not affected by treatment. Milk fat concentration was 12% lower, causing an 18% decrease in fat yield, when 180 g CLA·d-1 was infused. Concentration of trans 11-18:1 in blood plasma increased in proportion to CLA dose. TRAns 10-18:1 concentration in blood plasma also increased, and was 240% greater when CLA was infused at 180 g·d-1. Trans 10, cis12-18:2 was strictly a function of exogenous CLA input into the rumen, and ranged from 0.2 to 0.7 mg·g-1 of total plasma fatty acids. Yields of saturated 6:0 to 16:0 in milk fat decreased by 87 g·d-1 when 180 g CLA·d-1 was infused. Stearic acid concentration and yield increased by 25 and 6%, but cis9-18:1 yield decreased, in response to increasing dose of CLA. Yields of trans 11-18:1 and cis9,trans11-18:2 increased in proportion to CLA dose infused. Transfer rates of infused cis9,trans 11-18:2 or trans10,cis12-18:2 into milk fat averaged 3% at the highest dose of CLA infused. Milk fat yields of trans10-18:1 and trans10,cis12-18:2 also increased in proportion to CLA input. Lower normalized ratios of cis9-18:1 to 18:0 and cis9,trans11-18:2 to trans 11-18:1 in milk fat when CLA was infused suggested CLA reduced desaturation in the mammary gland. Results provide additional evidence that enhanced flow of trans 10-18:1 or trans 10,cis12-18:2 from the rumen may decrease milk fat yield by reducing de novo synthesis and desaturation.

Original languageEnglish (US)
Pages (from-to)463-475
Number of pages13
JournalAnimal Research
Volume50
Issue number6
StatePublished - Dec 1 2001
Externally publishedYes

Fingerprint

conjugated linoleic acid
blood plasma
Holstein
fatty acid composition
cows
milk fat
rumen
dosage
milk fat yield
milk fatty acids
hydrogenation
stearic acid
mammary glands
isomers
milk yield
fatty acids
milk
synthesis

Keywords

  • Cis9,trans11-18:2
  • Milk fat
  • Trans-vaccenic acid
  • Trans10,cis12-18:2
  • Trans10-18:1

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Alterations in blood plasma and milk fatty acid profiles of lactating Holstein cows in response to ruminal infusion of a conjugated linoleic acid mixture. / Loor, Juan J; Herbein, Joseph H.

In: Animal Research, Vol. 50, No. 6, 01.12.2001, p. 463-475.

Research output: Contribution to journalArticle

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