Allele-specific deposition of macroH2A1 in imprinting control regions

Jung Ha Choo, Jeong Do Kim, Jae Hoon Chung, Lisa Stubbs, Joomyeong Kim

Research output: Contribution to journalArticle

Abstract

In the current study, we analyzed the deposition patterns of macroH2A1 at a number of different genomic loci located in X chromosome and autosomes. MacroH2A1 is preferentially deposited at methylated CpG-rich regions located close to promoters. The macroH2A1 deposition patterns at the methylated CpG islands of several imprinted domains, including the imprinting control regions (ICRs) of Xist, Peg3, H19/Igf2, Gtl2/Dlk1 and Gnas domains, show consistent allele-specificity towards inactive, methylated alleles. The macroH2A1 deposition levels at the ICRs and other differentially methylated regions of these domains are also either higher or comparable to those observed at the inactive X chromosome of female mammals. Overall, our results indicate that besides DNA methylation macroH2A1 is another epigenetic component in the chromatin of ICRs displaying differential association with two parental alleles.

Original languageEnglish (US)
Pages (from-to)717-724
Number of pages8
JournalHuman molecular genetics
Volume15
Issue number5
DOIs
StatePublished - Mar 1 2006

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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