The release and characterization of alkaline phosphatases from a clinical and a non clinical strain of S. marcescens grown in two types of media were studied. Four different identifiable alkaline phosphatases (A', A, B, and E) were found to be releasable by polymyxin B treatment of whole cells grown in an inorganic phosphate free casamino acid medium, one of which, component B, was also releasable by saline wash from cells grown in a high inorganic phosphate glucose medium. SDS polyacrylamide gel electrophoresis followed by incubation of the gels in p nitrophenyl phosphate showed that there were three very active polymeric components (A', A, and B) with molecular weights of 190,000 ± 10,000, 140,000 ± 10,000, and 110,000 ± 10,000, respectively, and one possibly monomeric component, E, with molecular weight of 44,000 ± 1,000. Alkaline phosphatase B was present in both types of medium and was considered constitutive. However, the other two active alkaline phosphatases A', and A were present only in cells grown in the inorganic phosphate free casamino acid medium. By incubation at two temperatures (37°C and 70°C), prior to electrophoresis and enzyme assay, only component A remained active at 70°C. It is concluded that among the four alkaline phosphatases detected, one was a monomer (E), one was constitutive (B), and the remaining two (A' and A) were possibly inducible by an inorganic phosphate free medium. In addition, the effectiveness of polymyxin B on the release of periplasmic enzymes from cells of S. marcescens grown in different types of medium was discussed.
|Original language||English (US)|
|Number of pages||11|
|State||Published - Jan 1 1975|
ASJC Scopus subject areas
- Microbiology (medical)