TY - JOUR
T1 - Airyscan superresolution microscopy
T2 - A high-throughput alternative to electron microscopy for the visualization and analysis of fossil pollen
AU - Romero, Ingrid C.
AU - Urban, Michael A.
AU - Punyasena, Surangi W.
N1 - We thank Jorge Ceballos from Smithsonian Tropical Research Institute (STRI) for help with the SEM imaging; Lou Ann Miller from the Materials Research Laboratory at the University of Illinois (UIUC) for help with the TEM imaging; David Seigler from the UIUC Plant Biology Herbarium and James Solomon from Missouri Botanical Garden for providing herbarium specimens; Mayandi Sivaguru and Glenn Fried from the UIUC Carl R. Woese Institute for Genomic Biology for assistance with the Zeiss LSM 880 Airyscan microscope and feedback on the manuscript. We also thank Carlos Jaramillo from STRI and Francisca Oboh-Ikuenobe from the Missouri University of Science and Technology for sharing the fossil specimens and Doug Peterson and Michael Peterson for proofreading. Airyscan and TEM imaging was funded by NSF-DBI – Advances in Bioinformatics ( NSF-DBI-1262561 to SWP) and the Department of Plant Biology at UIUC .
We thank Jorge Ceballos from Smithsonian Tropical Research Institute (STRI) for help with the SEM imaging; Lou Ann Miller from the Materials Research Laboratory at the University of Illinois (UIUC) for help with the TEM imaging; David Seigler from the UIUC Plant Biology Herbarium and James Solomon from Missouri Botanical Garden for providing herbarium specimens; Mayandi Sivaguru and Glenn Fried from the UIUC Carl R. Woese Institute for Genomic Biology for assistance with the Zeiss LSM 880 Airyscan microscope and feedback on the manuscript. We also thank Carlos Jaramillo from STRI and Francisca Oboh-Ikuenobe from the Missouri University of Science and Technology for sharing the fossil specimens and Doug Peterson and Michael Peterson for proofreading. Airyscan and TEM imaging was funded by NSF-DBI ? Advances in Bioinformatics (NSF-DBI-1262561 to SWP) and the Department of Plant Biology at UIUC.
PY - 2020/5
Y1 - 2020/5
N2 - Airyscan confocal superresolution microscopy is a new optical technique that can detect morphological features smaller than the diffraction limit of light. It captures both the external and internal ornamentation of modern and fossil pollen. Airyscan combines the ease of use of optical microscopy methods, such as confocal and brightfield microscopy (BM), with the high-resolution imaging of electron microscopy (EM). Modern and fossil pollen grains were imaged using Airyscan, BM, and EM to assess the viability of Airyscan as an alternative for EM. Our results demonstrate that: (1) Airyscan can capture diagnostic features of extant and fossil pollen similar to EM; (2) one-to-one comparisons of Airyscan images with other optical methods are possible (e.g., BM and conventional confocal); (3) Airyscan captures three-dimensional data, allowing reconstruction of pollen grains in different views; and (4) the time and effort required for Airyscan imaging is significantly less than that for EM. This paper demonstrates that Airyscan superresolution microscopy is a high-throughput alternative for morphological analyses of pollen specimens.
AB - Airyscan confocal superresolution microscopy is a new optical technique that can detect morphological features smaller than the diffraction limit of light. It captures both the external and internal ornamentation of modern and fossil pollen. Airyscan combines the ease of use of optical microscopy methods, such as confocal and brightfield microscopy (BM), with the high-resolution imaging of electron microscopy (EM). Modern and fossil pollen grains were imaged using Airyscan, BM, and EM to assess the viability of Airyscan as an alternative for EM. Our results demonstrate that: (1) Airyscan can capture diagnostic features of extant and fossil pollen similar to EM; (2) one-to-one comparisons of Airyscan images with other optical methods are possible (e.g., BM and conventional confocal); (3) Airyscan captures three-dimensional data, allowing reconstruction of pollen grains in different views; and (4) the time and effort required for Airyscan imaging is significantly less than that for EM. This paper demonstrates that Airyscan superresolution microscopy is a high-throughput alternative for morphological analyses of pollen specimens.
KW - Comparative microscopy
KW - Confocal
KW - Pollen imaging
KW - Pollen ultrastructure
KW - Three-dimensional data
UR - https://www.scopus.com/pages/publications/85079399839
UR - https://www.scopus.com/inward/citedby.url?scp=85079399839&partnerID=8YFLogxK
U2 - 10.1016/j.revpalbo.2020.104192
DO - 10.1016/j.revpalbo.2020.104192
M3 - Article
AN - SCOPUS:85079399839
SN - 0034-6667
VL - 276
JO - Review of Palaeobotany and Palynology
JF - Review of Palaeobotany and Palynology
M1 - 104192
ER -