TY - JOUR
T1 - Agrobacterium-mediated transformation of Codonopsis lanceolata using the γ-TMT gene
AU - Ghimire, Bimal Kumar
AU - Seong, Eun Soo
AU - Lim, Jung Dae
AU - Heo, Kweon
AU - Kim, Myong Jo
AU - Chung, Ill Min
AU - Juvik, John A.
AU - Yu, Chang Yeon
N1 - Funding Information:
Fig. 7 Tocopherol contents in transgenic Codonopsis lanceolata plant: (a and b) HPLC profiles of tocopherol contents in the leaves collected from control (a) and transgenic (b) Codonopsis lanceolata plant; (c and d) HPLC profiles of Acknowledgements This research was conducted by the financial support of Bioherb Research Institute, Kangwon National University. This work was supported by the Korea Research Foundation grant funded by the Korea Government (MOEHRD) (KRF-2008-211-F00005) for the Returned Overseas superior Nepal scholars.
PY - 2008/12
Y1 - 2008/12
N2 - Efficient transformation of leaf disc-derived callus of Codonopsis lanceolata was obtained using Agrobacterium tumefaciens strain LBA4404 harboring a binary vector, pYBI121, that carries the neomycin phosphotransferase (npt II) gene as a selectable marker. The green shoots recovered from agroinfected explants on selection medium (containing 0.1 mg/l α-naphthaleneacetic acid (NAA), 1 mg/l 6-benzylaminopurine (BAP), 100 mg/l kanamycin, and 250 mg/l cefotaxime) were rooted on Murashige and Skoog (MS) medium supplemented with 2 mg/l IBA and 10 mg/l kanamycin. To optimize the transformation conditions, several factors were assessed, including the co-cultivation period, the duration of pre- and post-culture in darkness and light, the kanamycin concentration, and the Agrobacterium densities. We produced transgenic Codonopsis lanceolata overexpressing γ-tocopherol methyltransferase (γ-TMT) by this protocol. Moreover, the α-tocopherol content of the plants was enhanced by the overexpression of this gene.
AB - Efficient transformation of leaf disc-derived callus of Codonopsis lanceolata was obtained using Agrobacterium tumefaciens strain LBA4404 harboring a binary vector, pYBI121, that carries the neomycin phosphotransferase (npt II) gene as a selectable marker. The green shoots recovered from agroinfected explants on selection medium (containing 0.1 mg/l α-naphthaleneacetic acid (NAA), 1 mg/l 6-benzylaminopurine (BAP), 100 mg/l kanamycin, and 250 mg/l cefotaxime) were rooted on Murashige and Skoog (MS) medium supplemented with 2 mg/l IBA and 10 mg/l kanamycin. To optimize the transformation conditions, several factors were assessed, including the co-cultivation period, the duration of pre- and post-culture in darkness and light, the kanamycin concentration, and the Agrobacterium densities. We produced transgenic Codonopsis lanceolata overexpressing γ-tocopherol methyltransferase (γ-TMT) by this protocol. Moreover, the α-tocopherol content of the plants was enhanced by the overexpression of this gene.
KW - Agrobacterium tumefaciens
KW - Codonopsis lanceolata
KW - HPLC
KW - Regeneration
KW - Transformation
KW - Transgenic
KW - γ-Tocopherol
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U2 - 10.1007/s11240-008-9440-7
DO - 10.1007/s11240-008-9440-7
M3 - Article
AN - SCOPUS:54849410427
VL - 95
SP - 265
EP - 274
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 3
ER -