TY - JOUR
T1 - Age alters the observed response of imipramine binding sites to chronic antidepressant treatment in female rats
AU - Wilson, Marlene A.
AU - Roy, Edward J.
N1 - Funding Information:
This work was supported by NIMH grant MH33577 to EJ R and training grant funds from PHS-5-T32G M07143 to MAW. The authors would like to thank Diane M. Lynn and Vida
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1984/11/13
Y1 - 1984/11/13
N2 - Age alters hypothalamic imipramine binding sites in female rats. Older females have increased levels of imipramine binding with decreased affinity. The response of hypothalamic imipramine binding sites to chronic antidepressant treatment varies depending on the age of the animals treated. In juvenile female rats imipramine binding sites decrease after antidepressant treatment, consistent with previous reports. However, in young females chronic imipramine treatment produces no change in levels of imipramine binding with a slight decrease in binding affinity. In middle-aged female rats, the same treatment causes an apparent increase in levels of imipramine binding sites along with a decrease in affinity. These decreases in the affinity of imipramine binding sites in older female rats, and the concomitant changes in the levels of imipramine binding are the result of imipramine metabolites remaining in the tissue after antidepressant treatment and interfering with the binding assay, despite extensive washing of the membrane preparation. Thus, the observed changes in the response of imipramine binding sites to chronic imipramine treatment with age are probably due to age-related alterations in the metabolism of imipramine in female rats.
AB - Age alters hypothalamic imipramine binding sites in female rats. Older females have increased levels of imipramine binding with decreased affinity. The response of hypothalamic imipramine binding sites to chronic antidepressant treatment varies depending on the age of the animals treated. In juvenile female rats imipramine binding sites decrease after antidepressant treatment, consistent with previous reports. However, in young females chronic imipramine treatment produces no change in levels of imipramine binding with a slight decrease in binding affinity. In middle-aged female rats, the same treatment causes an apparent increase in levels of imipramine binding sites along with a decrease in affinity. These decreases in the affinity of imipramine binding sites in older female rats, and the concomitant changes in the levels of imipramine binding are the result of imipramine metabolites remaining in the tissue after antidepressant treatment and interfering with the binding assay, despite extensive washing of the membrane preparation. Thus, the observed changes in the response of imipramine binding sites to chronic imipramine treatment with age are probably due to age-related alterations in the metabolism of imipramine in female rats.
KW - Age
KW - Antidepressant treatment
KW - Imipramine binding sites
KW - Imipramine metabolism
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U2 - 10.1016/0014-2999(84)90726-X
DO - 10.1016/0014-2999(84)90726-X
M3 - Article
C2 - 6099275
AN - SCOPUS:0021742974
VL - 106
SP - 381
EP - 391
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 2
ER -