Abstract
Several high-resolution imaging techniques such as FESEM, TEM and AFM are compared with respect to their application on alginate hydrogels, a widely used polysaccharide biomaterial. A new AFM method applicable to RGD peptides covalently conjugated to alginate hydrogels is described. High-resolution images of RGD adhesion ligand distribution were obtained by labeling biotinylated RGD peptides with streptavidin-labeled gold nanoparticles. This method may broadly provide a useful tool for sECM characterization and design for tissue regeneration strategies. A figure is presented.
Original language | English (US) |
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Pages (from-to) | 469-477 |
Number of pages | 9 |
Journal | Macromolecular Bioscience |
Volume | 8 |
Issue number | 6 |
DOIs | |
State | Published - Jun 11 2008 |
Keywords
- Atomic force microscopy (AFM)
- Biomaterials
- Electron microscopy
- Hydrogels
- TEM
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomaterials
- Polymers and Plastics
- Materials Chemistry