AFM imaging of RGD presenting synthetic extracellular matrix using gold nanoparticles

Susan X. Hsiong, Peter H. Cooke, Hyun Joon Kong, Marshall L. Fishman, Maria Ericsson, David J. Mooney

Research output: Contribution to journalArticlepeer-review


Several high-resolution imaging techniques such as FESEM, TEM and AFM are compared with respect to their application on alginate hydrogels, a widely used polysaccharide biomaterial. A new AFM method applicable to RGD peptides covalently conjugated to alginate hydrogels is described. High-resolution images of RGD adhesion ligand distribution were obtained by labeling biotinylated RGD peptides with streptavidin-labeled gold nanoparticles. This method may broadly provide a useful tool for sECM characterization and design for tissue regeneration strategies. A figure is presented.

Original languageEnglish (US)
Pages (from-to)469-477
Number of pages9
JournalMacromolecular Bioscience
Issue number6
StatePublished - Jun 11 2008


  • Atomic force microscopy (AFM)
  • Biomaterials
  • Electron microscopy
  • Hydrogels
  • TEM

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry


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