AFLP analysis of genetic variability in New Guinea impatiens

Jason Carr, Mingliang Xu, John W. Dudley, Schuyler S. Korban

Research output: Contribution to journalArticlepeer-review


New Guinea impatiens (Impatiens hawkeri) is an economically important floral crop, however, little work has been conducted to further our understanding of the genetics of this crop. In this study, we used amplified fragment length polymorphism (AFLP) technology to investigate the level of polymorphism present among 41 commercial cultivars of New Guinea impatiens, study their genetic relatedness, and assess the genetic diversity in this material. An efficient DNA extraction protocol was developed, and a total of 48 EcoRI and MseI primer combinations were used for PCR amplification. Amplification products were then subjected to polyacrylamide gel electrophoresis. The AFLP analysis showed that all 41 cultivars generated between 73 and 130 scoreable polymorphic bands per primer combination. Gower's Genetic Dissimilarity estimates for the entire set of cultivars ranged between 0.940 and 0.488. A dendogram was generated from these dissimilarity data that revealed four groupings among these 41 cultivars. The implications of these results on genotypic variation, genetic relationships, and genetic diversity in New Guinea impatiens will be discussed.

Original languageEnglish (US)
Pages (from-to)1509-1516
Number of pages8
JournalTheoretical and Applied Genetics
Issue number8
StatePublished - May 1 2003


  • AFLP markers
  • Genetic analysis
  • Germplasm evaluation
  • Impatiens hawkeri

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics


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