Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

Jiazhang Lian, Mohammad HamediRad, Huimin Zhao

Research output: Contribution to journalReview article

Abstract

Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.

Original languageEnglish (US)
Article number1700601
JournalBiotechnology Journal
Volume13
Issue number9
DOIs
StatePublished - Sep 2018

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
Metabolic Engineering
Saccharomyces cerevisiae
Gene Knockout Techniques
Biotechnology
Transcriptional Activation
Technology

Keywords

  • Saccharomyces cerevisiae
  • gene regulation
  • genome engineering
  • metabolic engineering
  • synthetic biology

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

Cite this

Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System. / Lian, Jiazhang; HamediRad, Mohammad; Zhao, Huimin.

In: Biotechnology Journal, Vol. 13, No. 9, 1700601, 09.2018.

Research output: Contribution to journalReview article

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