Abstract
Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.
| Original language | English (US) |
|---|---|
| Article number | 1700601 |
| Journal | Biotechnology Journal |
| Volume | 13 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2018 |
Fingerprint
Keywords
- Saccharomyces cerevisiae
- gene regulation
- genome engineering
- metabolic engineering
- synthetic biology
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Molecular Medicine
Cite this
Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System. / Lian, Jiazhang; HamediRad, Mohammad; Zhao, Huimin.
In: Biotechnology Journal, Vol. 13, No. 9, 1700601, 09.2018.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System
AU - Lian, Jiazhang
AU - HamediRad, Mohammad
AU - Zhao, Huimin
PY - 2018/9
Y1 - 2018/9
N2 - Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.
AB - Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.
KW - Saccharomyces cerevisiae
KW - gene regulation
KW - genome engineering
KW - metabolic engineering
KW - synthetic biology
UR - http://www.scopus.com/inward/record.url?scp=85045741001&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045741001&partnerID=8YFLogxK
U2 - 10.1002/biot.201700601
DO - 10.1002/biot.201700601
M3 - Review article
C2 - 29436783
AN - SCOPUS:85045741001
VL - 13
JO - Biotechnology Journal
JF - Biotechnology Journal
SN - 1860-6768
IS - 9
M1 - 1700601
ER -