Abstract
Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.
| Original language | English (US) |
|---|---|
| Article number | 1700601 |
| Journal | Biotechnology Journal |
| Volume | 13 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2018 |
Keywords
- Saccharomyces cerevisiae
- gene regulation
- genome engineering
- metabolic engineering
- synthetic biology
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Molecular Medicine
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Cite this
Lian, J., HamediRad, M., & Zhao, H. (2018). Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System. Biotechnology Journal, 13(9), [1700601]. https://doi.org/10.1002/biot.201700601