Advances in targeted genome editing

Pablo Perez-Pinera, David G. Ousterout, Charles A. Gersbach

Research output: Contribution to journalReview article

Abstract

New technologies have recently emerged that enable targeted editing of genomes in diverse systems. This includes precise manipulation of gene sequences in their natural chromosomal context and addition of transgenes to specific genomic loci. This progress has been facilitated by advances in engineering targeted nucleases with programmable, site-specific DNA-binding domains, including zinc finger proteins and transcription activator-like effectors (TALEs). Recent improvements have enhanced nuclease performance, accelerated nuclease assembly, and lowered the cost of genome editing. These advances are driving new approaches to many areas of biotechnology, including biopharmaceutical production, agriculture, creation of transgenic organisms and cell lines, and studies of genome structure, regulation, and function. Genome editing is also being investigated in preclinical and clinical gene therapies for many diseases.

Original languageEnglish (US)
Pages (from-to)268-277
Number of pages10
JournalCurrent Opinion in Chemical Biology
Volume16
Issue number3-4
DOIs
StatePublished - Aug 1 2012
Externally publishedYes

Fingerprint

Genes
Genetically Modified Organisms
Zinc Fingers
Biotechnology
Agriculture
Transgenes
Genetic Therapy
Gene therapy
Genome
Technology
Costs and Cost Analysis
Cell Line
Transcription
DNA
Zinc
Cells
Gene Editing
Proteins
Costs
Transcription Activator-Like Effectors

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Advances in targeted genome editing. / Perez-Pinera, Pablo; Ousterout, David G.; Gersbach, Charles A.

In: Current Opinion in Chemical Biology, Vol. 16, No. 3-4, 01.08.2012, p. 268-277.

Research output: Contribution to journalReview article

Perez-Pinera, Pablo ; Ousterout, David G. ; Gersbach, Charles A. / Advances in targeted genome editing. In: Current Opinion in Chemical Biology. 2012 ; Vol. 16, No. 3-4. pp. 268-277.
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