Advances in multimodal mass spectrometry for single-cell analysis and imaging enhancement

Seth W. Croslow, Timothy J. Trinklein, Jonathan V. Sweedler

Research output: Contribution to journalArticlepeer-review


Multimodal mass spectrometry (MMS) incorporates an imaging modality with probe-based mass spectrometry (MS) to enable precise, targeted data acquisition and provide additional biological and chemical data not available by MS alone. Two categories of MMS are covered; in the first, an imaging modality guides the MS probe to target individual cells and to reduce acquisition time by automatically defining regions of interest. In the second category, imaging and MS data are coupled in the data analysis pipeline to increase the effective spatial resolution using a higher resolution imaging method, correct for tissue deformation, and incorporate fine morphological features in an MS imaging dataset. Recent methodological and computational developments are covered along with their application to single-cell and imaging analyses.

Original languageEnglish (US)
Pages (from-to)591-601
Number of pages11
JournalFEBS Letters
Issue number6
StatePublished - Mar 2024


  • cell
  • imaging
  • mass spectrometry
  • microscopy
  • multimodal
  • segmentation
  • subcellular

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biophysics
  • Structural Biology
  • Biochemistry
  • Cell Biology


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