Advances in imaging secondary ion mass spectrometry for biological samples

Steven G. Boxer, Mary L. Kraft, Peter K. Weber

Research output: Contribution to journalReview article

Abstract

Imaging mass spectrometry combines the power of mass spectrometry to identify complex molecules based on mass with sample imaging. Recent advances in secondary ion mass spectrometry have improved sensitivity and spatial resolution, so that these methods have the potential to bridge between high-resolution structures obtained by X-ray crystallography and cyro-electron microscopy and ultrastructure visualized by conventional light microscopy. Following background information on the method and instrumentation, we address the key issue of sample preparation. Because mass spectrometry is performed in high vacuum, it is essential to preserve the lateral organization of the sample while removing bulk water, and this has been a major barrier for applications to biological systems. Recent applications of imaging mass spectrometry to cell biology, microbial communities, and biosynthetic pathways are summarized briefly, and studies of biological membrane organization are described in greater depth.

Original languageEnglish (US)
Pages (from-to)53-74
Number of pages22
JournalAnnual Review of Biophysics
Volume38
Issue number1
DOIs
StatePublished - Nov 27 2009

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Secondary Ion Mass Spectrometry
Secondary ion mass spectrometry
Mass spectrometry
Mass Spectrometry
Imaging techniques
Cytology
Biological membranes
X ray crystallography
X Ray Crystallography
Biosynthetic Pathways
Biological systems
Vacuum
Electron microscopy
Optical microscopy
Cell Biology
Microscopy
Electron Microscopy
Light
Molecules
Membranes

Keywords

  • Chemical composition imaging
  • Dynamic SIMS
  • Membrane organization
  • NanoSIMS
  • ToF-SIMS

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Bioengineering
  • Biochemistry
  • Cell Biology

Cite this

Advances in imaging secondary ion mass spectrometry for biological samples. / Boxer, Steven G.; Kraft, Mary L.; Weber, Peter K.

In: Annual Review of Biophysics, Vol. 38, No. 1, 27.11.2009, p. 53-74.

Research output: Contribution to journalReview article

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