Advances in analyzing RNA diversity in eukaryotic transcriptomes: Peering through the Omics lens [version 1; referees: 3 approved]

Sushant Bangru, Auinash Kalsotra

Research output: Contribution to journalReview article

Abstract

Alternative splicing, polyadenylation, and chemical modifications of RNA generate astonishing complexity within eukaryotic transcriptomes. The last decade has brought numerous advances in sequencing technologies that allow biologists to investigate these phenomena with greater depth and accuracy while reducing time and cost. A commensurate development in biochemical techniques for the enrichment and analysis of different RNA variants has accompanied the advancement of global sequencing analysis platforms. Here, we present a detailed overview of the latest biochemical methods, along with bioinformatics pipelines that have aided in identifying different RNA variants. We also highlight the ongoing developments and challenges associated with RNA variant detection and quantification, including sample heterogeneity and isolation, as well as 'Omics' big data handling.

Original languageEnglish (US)
Article number2668
JournalF1000Research
Volume5
DOIs
StatePublished - Jan 1 2016

Fingerprint

Transcriptome
Lenses
RNA
Polyadenylation
Data handling
Chemical modification
Alternative Splicing
Bioinformatics
Computational Biology
Pipelines
Technology
Costs and Cost Analysis
Costs

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Advances in analyzing RNA diversity in eukaryotic transcriptomes : Peering through the Omics lens [version 1; referees: 3 approved]. / Bangru, Sushant; Kalsotra, Auinash.

In: F1000Research, Vol. 5, 2668, 01.01.2016.

Research output: Contribution to journalReview article

@article{ed5d6fc8860b49b7ac4f15fd0613f31c,
title = "Advances in analyzing RNA diversity in eukaryotic transcriptomes: Peering through the Omics lens [version 1; referees: 3 approved]",
abstract = "Alternative splicing, polyadenylation, and chemical modifications of RNA generate astonishing complexity within eukaryotic transcriptomes. The last decade has brought numerous advances in sequencing technologies that allow biologists to investigate these phenomena with greater depth and accuracy while reducing time and cost. A commensurate development in biochemical techniques for the enrichment and analysis of different RNA variants has accompanied the advancement of global sequencing analysis platforms. Here, we present a detailed overview of the latest biochemical methods, along with bioinformatics pipelines that have aided in identifying different RNA variants. We also highlight the ongoing developments and challenges associated with RNA variant detection and quantification, including sample heterogeneity and isolation, as well as 'Omics' big data handling.",
author = "Sushant Bangru and Auinash Kalsotra",
year = "2016",
month = "1",
day = "1",
doi = "10.12688/f1000research.9511.1",
language = "English (US)",
volume = "5",
journal = "F1000Research",
issn = "2046-1402",
publisher = "F1000 Research Ltd.",

}

TY - JOUR

T1 - Advances in analyzing RNA diversity in eukaryotic transcriptomes

T2 - Peering through the Omics lens [version 1; referees: 3 approved]

AU - Bangru, Sushant

AU - Kalsotra, Auinash

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Alternative splicing, polyadenylation, and chemical modifications of RNA generate astonishing complexity within eukaryotic transcriptomes. The last decade has brought numerous advances in sequencing technologies that allow biologists to investigate these phenomena with greater depth and accuracy while reducing time and cost. A commensurate development in biochemical techniques for the enrichment and analysis of different RNA variants has accompanied the advancement of global sequencing analysis platforms. Here, we present a detailed overview of the latest biochemical methods, along with bioinformatics pipelines that have aided in identifying different RNA variants. We also highlight the ongoing developments and challenges associated with RNA variant detection and quantification, including sample heterogeneity and isolation, as well as 'Omics' big data handling.

AB - Alternative splicing, polyadenylation, and chemical modifications of RNA generate astonishing complexity within eukaryotic transcriptomes. The last decade has brought numerous advances in sequencing technologies that allow biologists to investigate these phenomena with greater depth and accuracy while reducing time and cost. A commensurate development in biochemical techniques for the enrichment and analysis of different RNA variants has accompanied the advancement of global sequencing analysis platforms. Here, we present a detailed overview of the latest biochemical methods, along with bioinformatics pipelines that have aided in identifying different RNA variants. We also highlight the ongoing developments and challenges associated with RNA variant detection and quantification, including sample heterogeneity and isolation, as well as 'Omics' big data handling.

UR - http://www.scopus.com/inward/record.url?scp=85020002782&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020002782&partnerID=8YFLogxK

U2 - 10.12688/f1000research.9511.1

DO - 10.12688/f1000research.9511.1

M3 - Review article

AN - SCOPUS:85020002782

VL - 5

JO - F1000Research

JF - F1000Research

SN - 2046-1402

M1 - 2668

ER -