Abstract
Until recently, there were no generalizable methods for assessing the effects of post-translational regulation on enzymatic activity. Activity-based sensing (ABS) has emerged as a powerful approach for monitoring small-molecule and enzyme activities within living systems. Initial examples of ABS were applied for measuring general enzymatic activity; however, a recent focus has been placed on increasing the selectivity to monitor a single enzyme or isoform. The highest degree of selectivity is required for differentiating between isoforms, where the targets display significant structural similarities as a result of a gene duplication or alternative splicing. This Minireview highlights key examples of small-molecule isoform-selective probes with a focus on the relevance of isoform differentiation, design strategies to achieve selectivity, and applications in basic biology or in the clinic.
Original language | English (US) |
---|---|
Journal | Angewandte Chemie - International Edition |
DOIs | |
State | Accepted/In press - 2020 |
Keywords
- activity-based sensing
- fluorescence
- molecular imaging
- probe development
- rational design
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)