TY - JOUR
T1 - Advanced Glycation End Products and Inflammatory Cytokine Profiles in Maintenance Hemodialysis Patients After the Ingestion of a Protein-Dense Meal
AU - Perkins, Ryan K.
AU - van Vliet, Stephan
AU - Miranda, Edwin R.
AU - Fuller, Kelly N.Z.
AU - Beisswenger, Paul J.
AU - Wilund, Kenneth R.
AU - Paluska, Scott A.
AU - Burd, Nicholas A.
AU - Haus, Jacob M.
N1 - Support: This research was supported by a grant from the American Egg Board/Egg Nutrition Center, United States and National Institutes of Health, United States R01 DK109948. SvV was supported by a European Society for Clinical Nutrition and Metabolism Research Fellowship. Clinical Trial Registration: NCT03478722 (www.clincialtrials.gov/). Financial Disclosure: The authors declare that they have no relevant financial interests.
Support: This research was supported by a grant from the American Egg Board /Egg Nutrition Center, United States and National Institutes of Health, United States R01 DK109948. SvV was supported by a European Society for Clinical Nutrition and Metabolism Research Fellowship. Clinical Trial Registration: NCT03478722 ( www.clincialtrials.gov/ ).
PY - 2023/1
Y1 - 2023/1
N2 - Objective: The goal of this investigation was to evaluate circulating and skeletal muscle inflammatory biomarkers between maintenance hemodialysis (MHD) and demographic-matched control subjects (CON) before and after ingestion of a protein-rich meal. Design and Methods: CON (n = 8; 50 ± 2 years; 31 ± 1 kg/m2) and MHD patients (n = 8; 56 ± 5 years; 32 ± 2 kg/m2) underwent a basal blood draw and muscle biopsy and serial blood draws after the ingestion of a mixed meal on a nondialysis day. Plasma advanced glycation end products (AGEs) and markers of oxidation were assessed via liquid chromatography–tandem mass spectrometry before and after the meal (+240 min). Circulating inflammatory cytokines and soluble receptors for AGE (sRAGE) isoforms (endogenous secretory RAGEs and cleaved RAGEs) were determined before and after the meal (+240 min). Basal muscle was probed for inflammatory cytokines and protein expression of related signaling components (RAGE, Toll-like receptor 4, oligosaccharyltransferase subunit 48, TIR-domain–containing adapter-inducing interferon-β, total IκBα, and pIκBα). Results: Basal circulating AGEs were 7- to 343-fold higher (P < .001) in MHD than those in CON, but only MG-H1 increased in CON after the meal (P < .001). There was a group effect (MHD > CON) for total sRAGEs (P = .02) and endogenous secretory RAGEs (P < .001) and a trend for cleaved RAGEs (P=.09), with no meal effect. In addition, there was a group effect (MHD < CON; P < .05) for circulating fractalkine, interleukin (IL)10, IL17A, and IL1β and a trend (P < .10) for IL6 and macrophage inflammatory protein 1 alpha, whereas tumor necrosis factor alpha was higher in MHD (P < .001). In muscle, Toll-like receptor 4 (P = .03), TIR-domain–containing adapter-inducing interferon-β (P = .002), and oligosaccharyltransferase subunit 48 (P = .02) expression was lower in MHD than that in CON, whereas IL6 was higher (P = .01) and IL8 (P = .08) tended to be higher in MHD. Conclusion: Overall, MHD exhibited an exaggerated, circulating, and skeletal muscle inflammatory biomarker environment, and the meal did not appreciably affect the inflammatory status.
AB - Objective: The goal of this investigation was to evaluate circulating and skeletal muscle inflammatory biomarkers between maintenance hemodialysis (MHD) and demographic-matched control subjects (CON) before and after ingestion of a protein-rich meal. Design and Methods: CON (n = 8; 50 ± 2 years; 31 ± 1 kg/m2) and MHD patients (n = 8; 56 ± 5 years; 32 ± 2 kg/m2) underwent a basal blood draw and muscle biopsy and serial blood draws after the ingestion of a mixed meal on a nondialysis day. Plasma advanced glycation end products (AGEs) and markers of oxidation were assessed via liquid chromatography–tandem mass spectrometry before and after the meal (+240 min). Circulating inflammatory cytokines and soluble receptors for AGE (sRAGE) isoforms (endogenous secretory RAGEs and cleaved RAGEs) were determined before and after the meal (+240 min). Basal muscle was probed for inflammatory cytokines and protein expression of related signaling components (RAGE, Toll-like receptor 4, oligosaccharyltransferase subunit 48, TIR-domain–containing adapter-inducing interferon-β, total IκBα, and pIκBα). Results: Basal circulating AGEs were 7- to 343-fold higher (P < .001) in MHD than those in CON, but only MG-H1 increased in CON after the meal (P < .001). There was a group effect (MHD > CON) for total sRAGEs (P = .02) and endogenous secretory RAGEs (P < .001) and a trend for cleaved RAGEs (P=.09), with no meal effect. In addition, there was a group effect (MHD < CON; P < .05) for circulating fractalkine, interleukin (IL)10, IL17A, and IL1β and a trend (P < .10) for IL6 and macrophage inflammatory protein 1 alpha, whereas tumor necrosis factor alpha was higher in MHD (P < .001). In muscle, Toll-like receptor 4 (P = .03), TIR-domain–containing adapter-inducing interferon-β (P = .002), and oligosaccharyltransferase subunit 48 (P = .02) expression was lower in MHD than that in CON, whereas IL6 was higher (P = .01) and IL8 (P = .08) tended to be higher in MHD. Conclusion: Overall, MHD exhibited an exaggerated, circulating, and skeletal muscle inflammatory biomarker environment, and the meal did not appreciably affect the inflammatory status.
KW - AGEs
KW - Diet
KW - Inflammation
KW - Maintenance Hemodialysis
UR - https://www.scopus.com/pages/publications/85123356177
UR - https://www.scopus.com/pages/publications/85123356177#tab=citedBy
U2 - 10.1053/j.jrn.2021.11.006
DO - 10.1053/j.jrn.2021.11.006
M3 - Article
C2 - 34923111
AN - SCOPUS:85123356177
SN - 1051-2276
VL - 33
SP - 181
EP - 192
JO - Journal of Renal Nutrition
JF - Journal of Renal Nutrition
IS - 1
ER -