Adsorption kinetics and reversibility of linear plasmid DNA on silica surfaces: Influence of alkaline earth and transition metal ions

Thanh Huong Nguyen, Kai Loon Chen, Menachem Elimelech

Research output: Contribution to journalArticlepeer-review

Abstract

A quartz crystal microbalance with dissipation monitoring is used to study the adsorption of linear plasmid DNA on silica surfaces and silica surfaces coated with poly-l-lysine (PLL) in solutions containing either alkaline earth (calcium and magnesium) or transition (cobalt, copper, and zinc) metals. Our results show that electrostatic attraction alone does not fully explain the significantly higher adsorption rate of DNA on the positively charged PLL layer in Cu2+ solution compared to solutions containing Ca2+, Mg2+, Co2+, or Zn2+. Diffusion coefficients measured by dynamic light scattering reveal that the compactness of plasmid DNA molecules is greater in solutions containing Cu2+ compared to that of DNA in other divalent electrolyte solutions. When the adsorption rate of plasmid DNA on silica is normalized to the corresponding adsorption rate on PLL-coated surfaces at the same solution condition, the attachment (adsorption) efficiencies are about 0.01 for Ca2+ or Mg2, but close to unity for Co2+, Cu2+, or Zn2+. Results from viscoelastic modeling of adsorbed DNA layers suggest that the DNA layer formed in Cu2+ solutions is thicker and more viscous compared to that formed in Co2+ solutions. This study demonstrates that plasmid DNA has a strong affinity to Cu2+, which results in a more compact conformation of DNA molecules compared to the case with the other divalent cations investigated.

Original languageEnglish (US)
Pages (from-to)1225-1230
Number of pages6
JournalBiomacromolecules
Volume11
Issue number5
DOIs
StatePublished - May 10 2010

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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