Adipose tissue transcriptome changes during obesity development in female dogs

Ryan W. Grant, Brittany M. Vester Boler, Tonya K. Ridge, Thomas K. Graves, Kelly S. Swanson

Research output: Contribution to journalArticle

Abstract

During the development of obesity, adipose tissue undergoes major expansion and remodeling, but the biological processes involved in this transition are not well understood. The objective of this study was to analyze global gene expression profiles of adipose tissue in dogs, fed a high-fat diet, during the transition from a lean to obese phenotype. Nine female beagles (4.09 ± 0.64 yr; 8.48 ± 0.35 kg) were randomized to ad libitum feeding or body weight maintenance. Subcutaneous adipose tissue biopsy, blood, and dual x-ray absorptiometry measurements were collected at 0, 4, 8, 12, and 24 wk of feeding. Serum was analyzed for glucose, insulin, fruc- tosamine, triglycerides, free fatty acids, adiponectin, and leptin. Forma- lin-fixed adipose tissue was used for determination of adipocyte size. Adipose RNA samples were hybridized to Affymetrix Canine 2.0 mi- croarrays. Statistical analysis, using repeated-measures ANOVA, showed ad libitum feeding increased (P < 0.05) body weight (0 wk, 8.36 ± 0.34 kg; 24 wk, 14.64 ± 34 kg), body fat mass (0 wk, 1.36 ± 024 kg; 24 wk, 6.52 ± 0.24 kg), adipocyte size (0 wk, 114.66 ± 17.38μm2; 24 wk, 320.97 ± 0.18.17 μm2), and leptin (0 wk, 0.8 ± 1.0 ng/ml; 24 wk, 12.9 ± 1.0ng/ml). Microarrays displayed 1,665 differentially expressed genes in adipose tissue as weight increased. Alterations were seen in adipose tissue homeostatic processes including metabolism, oxidative stress, mitochondrial homeostasis, and extracellular matrix. Adipose transcriptome changes highlight the dynamic and adaptive response to ad libitum feeding and obesity development.

Original languageEnglish (US)
Pages (from-to)295-307
Number of pages13
JournalPhysiological genomics
Volume43
Issue number6
DOIs
StatePublished - Mar 1 2011

Keywords

  • Extracellular matrix
  • Gene expression
  • Metabolism
  • Oxidation

ASJC Scopus subject areas

  • Physiology
  • Genetics

Fingerprint Dive into the research topics of 'Adipose tissue transcriptome changes during obesity development in female dogs'. Together they form a unique fingerprint.

  • Cite this