Adipose-derived mesenchymal stem cells enhance healing of mandibular defects in the ramus of swine

Shanna M. Wilson, Michael S. Goldwasser, Sherrie G. Clark, Elisa Monaco, Massimo Bionaz, Walter L. Hurley, Sandra Luisa Rodriguez-Zas, Liang Feng, Zaneta Dymon, Matthew B Wheeler

Research output: Contribution to journalArticle

Abstract

Purpose: This study investigated the effect of adipose-derived mesenchymal stem cells (ASCs) injected locally or systemically on the bone regeneration of a 10-mm-diameter cylindrical noncritical-size defect in the ramus of the pig mandible. Materials and Methods: Fifteen Yorkshire pigs, weighing 60 to 80 kg, received bilateral 10-mmdiameter cylindrical surgical defects in each ramus of the mandible. Pigs received 1) a direct injection into the defect of 2.5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); 2) an ear vein injection of 5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); or 3) an ear vein injection of culture Dulbecco's Modified Eagle's Medium without stem cells (control; n = 3). Pigs from each treatment were sacrificed at 1 hour, 2 weeks, or 4 weeks after surgery. Healing of the defect was evaluated by dual-energy x-ray absorptiometry, micro-computed tomography, fluorescent microscopy, and histology. Results: Bone healing was accelerated in the ASC-injected treatment groups at 2 and 4 weeks after surgery compared with the control pigs. Conclusions: Results from this animal model provide evidence that the injection of ASC locally into a bone defect or systemically can accelerate the healing of bone.

Original languageEnglish (US)
JournalJournal of Oral and Maxillofacial Surgery
Volume70
Issue number3
DOIs
StatePublished - Mar 2012

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Mesenchymal Stromal Cells
Swine
Injections
Mandible
Bone and Bones
Ear
Veins
Esters
Eagles
Bone Regeneration
Microscopy
Histology
Stem Cells
Animal Models
Tomography
X-Rays
Therapeutics

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology

Cite this

Adipose-derived mesenchymal stem cells enhance healing of mandibular defects in the ramus of swine. / Wilson, Shanna M.; Goldwasser, Michael S.; Clark, Sherrie G.; Monaco, Elisa; Bionaz, Massimo; Hurley, Walter L.; Rodriguez-Zas, Sandra Luisa; Feng, Liang; Dymon, Zaneta; Wheeler, Matthew B.

In: Journal of Oral and Maxillofacial Surgery, Vol. 70, No. 3, 03.2012.

Research output: Contribution to journalArticle

Wilson, Shanna M. ; Goldwasser, Michael S. ; Clark, Sherrie G. ; Monaco, Elisa ; Bionaz, Massimo ; Hurley, Walter L. ; Rodriguez-Zas, Sandra Luisa ; Feng, Liang ; Dymon, Zaneta ; Wheeler, Matthew B. / Adipose-derived mesenchymal stem cells enhance healing of mandibular defects in the ramus of swine. In: Journal of Oral and Maxillofacial Surgery. 2012 ; Vol. 70, No. 3.
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