TY - JOUR
T1 - Adipose-derived mesenchymal stem cells enhance healing of mandibular defects in the ramus of swine
AU - Wilson, Shanna M.
AU - Goldwasser, Michael S.
AU - Clark, Sherrie G.
AU - Monaco, Elisa
AU - Bionaz, Massimo
AU - Hurley, Walter L.
AU - Rodriguez-Zas, Sandra
AU - Feng, Liang
AU - Dymon, Zaneta
AU - Wheeler, Matthew B.
PY - 2012/3
Y1 - 2012/3
N2 - Purpose: This study investigated the effect of adipose-derived mesenchymal stem cells (ASCs) injected locally or systemically on the bone regeneration of a 10-mm-diameter cylindrical noncritical-size defect in the ramus of the pig mandible. Materials and Methods: Fifteen Yorkshire pigs, weighing 60 to 80 kg, received bilateral 10-mmdiameter cylindrical surgical defects in each ramus of the mandible. Pigs received 1) a direct injection into the defect of 2.5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); 2) an ear vein injection of 5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); or 3) an ear vein injection of culture Dulbecco's Modified Eagle's Medium without stem cells (control; n = 3). Pigs from each treatment were sacrificed at 1 hour, 2 weeks, or 4 weeks after surgery. Healing of the defect was evaluated by dual-energy x-ray absorptiometry, micro-computed tomography, fluorescent microscopy, and histology. Results: Bone healing was accelerated in the ASC-injected treatment groups at 2 and 4 weeks after surgery compared with the control pigs. Conclusions: Results from this animal model provide evidence that the injection of ASC locally into a bone defect or systemically can accelerate the healing of bone.
AB - Purpose: This study investigated the effect of adipose-derived mesenchymal stem cells (ASCs) injected locally or systemically on the bone regeneration of a 10-mm-diameter cylindrical noncritical-size defect in the ramus of the pig mandible. Materials and Methods: Fifteen Yorkshire pigs, weighing 60 to 80 kg, received bilateral 10-mmdiameter cylindrical surgical defects in each ramus of the mandible. Pigs received 1) a direct injection into the defect of 2.5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); 2) an ear vein injection of 5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); or 3) an ear vein injection of culture Dulbecco's Modified Eagle's Medium without stem cells (control; n = 3). Pigs from each treatment were sacrificed at 1 hour, 2 weeks, or 4 weeks after surgery. Healing of the defect was evaluated by dual-energy x-ray absorptiometry, micro-computed tomography, fluorescent microscopy, and histology. Results: Bone healing was accelerated in the ASC-injected treatment groups at 2 and 4 weeks after surgery compared with the control pigs. Conclusions: Results from this animal model provide evidence that the injection of ASC locally into a bone defect or systemically can accelerate the healing of bone.
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U2 - 10.1016/j.joms.2011.10.029
DO - 10.1016/j.joms.2011.10.029
M3 - Article
C2 - 22374062
AN - SCOPUS:84859934323
SN - 0278-2391
VL - 70
SP - e193-e203
JO - Journal of Oral and Maxillofacial Surgery
JF - Journal of Oral and Maxillofacial Surgery
IS - 3
ER -