Adipose-derived mesenchymal stem cells enhance healing of mandibular defects in the ramus of swine

Shanna M. Wilson, Michael S. Goldwasser, Sherrie G. Clark, Elisa Monaco, Massimo Bionaz, Walter L. Hurley, Sandra Rodriguez-Zas, Liang Feng, Zaneta Dymon, Matthew B. Wheeler

Research output: Contribution to journalArticle

Abstract

Purpose: This study investigated the effect of adipose-derived mesenchymal stem cells (ASCs) injected locally or systemically on the bone regeneration of a 10-mm-diameter cylindrical noncritical-size defect in the ramus of the pig mandible. Materials and Methods: Fifteen Yorkshire pigs, weighing 60 to 80 kg, received bilateral 10-mmdiameter cylindrical surgical defects in each ramus of the mandible. Pigs received 1) a direct injection into the defect of 2.5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); 2) an ear vein injection of 5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); or 3) an ear vein injection of culture Dulbecco's Modified Eagle's Medium without stem cells (control; n = 3). Pigs from each treatment were sacrificed at 1 hour, 2 weeks, or 4 weeks after surgery. Healing of the defect was evaluated by dual-energy x-ray absorptiometry, micro-computed tomography, fluorescent microscopy, and histology. Results: Bone healing was accelerated in the ASC-injected treatment groups at 2 and 4 weeks after surgery compared with the control pigs. Conclusions: Results from this animal model provide evidence that the injection of ASC locally into a bone defect or systemically can accelerate the healing of bone.

Original languageEnglish (US)
Pages (from-to)e193-e203
JournalJournal of Oral and Maxillofacial Surgery
Volume70
Issue number3
DOIs
StatePublished - Mar 2012

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology

Fingerprint Dive into the research topics of 'Adipose-derived mesenchymal stem cells enhance healing of mandibular defects in the ramus of swine'. Together they form a unique fingerprint.

  • Cite this