Adhesion studies of single living cells using MEMS sensors

Chad Sager, Taher Saif, Phil LeDuc

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Adhesion between cells is related to several physiological phenomena such as heart failure (KarilaOO), how cancer spreads (Ruoslahti99) and if an infection will be fought off. Controlling of these events requires knowledge of how cells adhere. Many previous studies have been conducted with various amounts of success. But none of these methods are independently capable of understanding the adhesion properties of a single living cell. In this article a MEMS sensor has been employed to study, quantitatively and qualitatively, the adhesion properties of a single living bovine endothelial cell. This experiment shows that the strength of a single anchorage site of the endothelial cell to an extracellular matrix coated substrate is 36 nN. Anchorage sites have been observed, in-situ, to be spaced on the order of 1 μm intervals. A model is also proposed for the detachment of a single living cell from a substrate.

Original languageEnglish (US)
Title of host publicationMicro-Electro-Mechanical Systems (MEMS)
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages215-219
Number of pages5
ISBN (Electronic)9780791819005
DOIs
StatePublished - 2000
EventASME 2000 International Mechanical Engineering Congress and Exposition, IMECE 2000 - Orlando, United States
Duration: Nov 5 2000Nov 10 2000

Publication series

NameASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
Volume2000-AB

Conference

ConferenceASME 2000 International Mechanical Engineering Congress and Exposition, IMECE 2000
Country/TerritoryUnited States
CityOrlando
Period11/5/0011/10/00

ASJC Scopus subject areas

  • Mechanical Engineering

Fingerprint

Dive into the research topics of 'Adhesion studies of single living cells using MEMS sensors'. Together they form a unique fingerprint.

Cite this