TY - GEN
T1 - Adhesion studies of single living cells using MEMS sensors
AU - Sager, Chad
AU - Saif, Taher
AU - LeDuc, Phil
N1 - Publisher Copyright:
Copyright © 2000 by ASME
PY - 2000
Y1 - 2000
N2 - Adhesion between cells is related to several physiological phenomena such as heart failure (KarilaOO), how cancer spreads (Ruoslahti99) and if an infection will be fought off. Controlling of these events requires knowledge of how cells adhere. Many previous studies have been conducted with various amounts of success. But none of these methods are independently capable of understanding the adhesion properties of a single living cell. In this article a MEMS sensor has been employed to study, quantitatively and qualitatively, the adhesion properties of a single living bovine endothelial cell. This experiment shows that the strength of a single anchorage site of the endothelial cell to an extracellular matrix coated substrate is 36 nN. Anchorage sites have been observed, in-situ, to be spaced on the order of 1 μm intervals. A model is also proposed for the detachment of a single living cell from a substrate.
AB - Adhesion between cells is related to several physiological phenomena such as heart failure (KarilaOO), how cancer spreads (Ruoslahti99) and if an infection will be fought off. Controlling of these events requires knowledge of how cells adhere. Many previous studies have been conducted with various amounts of success. But none of these methods are independently capable of understanding the adhesion properties of a single living cell. In this article a MEMS sensor has been employed to study, quantitatively and qualitatively, the adhesion properties of a single living bovine endothelial cell. This experiment shows that the strength of a single anchorage site of the endothelial cell to an extracellular matrix coated substrate is 36 nN. Anchorage sites have been observed, in-situ, to be spaced on the order of 1 μm intervals. A model is also proposed for the detachment of a single living cell from a substrate.
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U2 - 10.1115/IMECE2000-1094
DO - 10.1115/IMECE2000-1094
M3 - Conference contribution
AN - SCOPUS:0242527653
T3 - ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
SP - 215
EP - 219
BT - Micro-Electro-Mechanical Systems (MEMS)
PB - American Society of Mechanical Engineers (ASME)
T2 - ASME 2000 International Mechanical Engineering Congress and Exposition, IMECE 2000
Y2 - 5 November 2000 through 10 November 2000
ER -