Abstract
We previously used in vitro selection to identify the 7S11 deoxyribozyme, which catalyzes formation of 2′,5′-branched RNA using a branch-site adenosine nucleophile and a 5′-triphosphate electrophile. An unanswered question is whether the use of branch-site adenosine is inherently preferred or a chance event during the particular selection experiment. Here we have found that deoxyribozymes newly selected to use uridine as the branch-site RNA nucleotide in a structural context that resembles natural RNA splicing instead prefer a branch-site adenosine, although adenosine was never available during the selection itself. Our results support a chemical basis for nature's choice of the branch-site nucleotide, which is almost always adenosine in group II introns and the spliceosome.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2767-2771 |
| Number of pages | 5 |
| Journal | Biochemistry |
| Volume | 45 |
| Issue number | 9 |
| DOIs | |
| State | Published - Mar 7 2006 |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Adenosine is inherently favored as the branch-site RNA nucleotide in a structural context that resembles natural RNA splicing. / Zelin, Elena; Wang, Yangming; Silverman, Scott K.
In: Biochemistry, Vol. 45, No. 9, 07.03.2006, p. 2767-2771.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Adenosine is inherently favored as the branch-site RNA nucleotide in a structural context that resembles natural RNA splicing
AU - Zelin, Elena
AU - Wang, Yangming
AU - Silverman, Scott K
PY - 2006/3/7
Y1 - 2006/3/7
N2 - We previously used in vitro selection to identify the 7S11 deoxyribozyme, which catalyzes formation of 2′,5′-branched RNA using a branch-site adenosine nucleophile and a 5′-triphosphate electrophile. An unanswered question is whether the use of branch-site adenosine is inherently preferred or a chance event during the particular selection experiment. Here we have found that deoxyribozymes newly selected to use uridine as the branch-site RNA nucleotide in a structural context that resembles natural RNA splicing instead prefer a branch-site adenosine, although adenosine was never available during the selection itself. Our results support a chemical basis for nature's choice of the branch-site nucleotide, which is almost always adenosine in group II introns and the spliceosome.
AB - We previously used in vitro selection to identify the 7S11 deoxyribozyme, which catalyzes formation of 2′,5′-branched RNA using a branch-site adenosine nucleophile and a 5′-triphosphate electrophile. An unanswered question is whether the use of branch-site adenosine is inherently preferred or a chance event during the particular selection experiment. Here we have found that deoxyribozymes newly selected to use uridine as the branch-site RNA nucleotide in a structural context that resembles natural RNA splicing instead prefer a branch-site adenosine, although adenosine was never available during the selection itself. Our results support a chemical basis for nature's choice of the branch-site nucleotide, which is almost always adenosine in group II introns and the spliceosome.
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U2 - 10.1021/bi052499l
DO - 10.1021/bi052499l
M3 - Article
VL - 45
SP - 2767
EP - 2771
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 9
ER -