Adenosine A2A receptors assemble into higher-order oligomers at the plasma membrane

Pierre Alexandre Vidi, Jiji Chen, Joseph M.K. Irudayaraj, Val J. Watts

Research output: Contribution to journalArticle

Abstract

Oligomerization of G protein-coupled receptors (GPCRs) is known to play important roles in regulating receptor pharmacology and function. Whereas many bivalent GPCR interactions have been described, the stoichiometry and localization of GPCR oligomers are largely unknown. We have used bimolecular fluorescence complementation (BiFC) to study adenosine A2A receptor (A2AR) oligomerization. The data suggest specificity of the A2AR/A2AR interaction monitored by BiFC and proper sub-cellular localization of tagged receptors. Moreover, using a novel approach combining fluorescence resonance energy transfer and BiFC, we found that at least three A2A receptors assemble into higher-order oligomers at the plasma membrane in Cath.A differentiated neuronal cells. Structured summary: MINT-6797156, MINT-6797142: A2AR (uniprotkb:P29274) physically interacts (MI:0218) with A2AR (uniprotkb:P29274) by bimolecular fluorescence complementation (MI:0809)MINT-6797129: A2AR (uniprotkb:P29274) physically interacts (MI:0218) with A2AR (uniprotkb:P29274) by fluorescent resonance energy transfer (MI:0055).

Original languageEnglish (US)
Pages (from-to)3985-3990
Number of pages6
JournalFEBS Letters
Volume582
Issue number29
DOIs
StatePublished - Dec 10 2008
Externally publishedYes

Fingerprint

Adenosine A2A Receptors
Cell membranes
Oligomers
G-Protein-Coupled Receptors
Fluorescence
Cell Membrane
Oligomerization
Fluorescence Resonance Energy Transfer
Energy Transfer
Stoichiometry
Energy transfer
Pharmacology

Keywords

  • Adenosine A receptor
  • Bimolecular fluorescence complementation
  • Fluorescence (Forster) resonance energy transfer
  • G protein-coupled receptor
  • Higher-order oligomer
  • Neuronal cell

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Adenosine A2A receptors assemble into higher-order oligomers at the plasma membrane. / Vidi, Pierre Alexandre; Chen, Jiji; Irudayaraj, Joseph M.K.; Watts, Val J.

In: FEBS Letters, Vol. 582, No. 29, 10.12.2008, p. 3985-3990.

Research output: Contribution to journalArticle

Vidi, Pierre Alexandre ; Chen, Jiji ; Irudayaraj, Joseph M.K. ; Watts, Val J. / Adenosine A2A receptors assemble into higher-order oligomers at the plasma membrane. In: FEBS Letters. 2008 ; Vol. 582, No. 29. pp. 3985-3990.
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