Addition of polydextrose and galactooligosaccharide to formula does not affect bacterial translocation in the neonatal piglet

Marcia H. Monaco, Dimitri O. Kashtanov, Mei Wang, D. Carey Walker, Deshanie Rai, Zeina E. Jouni, Michael J. Miller, Sharon M. Donovan

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: The aim of the study was to determine the effect of polydextrose (PDX) and galactooligosaccharide (GOS) on bacterial translocation (BT) in neonatal piglets. Materials and Methods: Piglets (n = 36) were randomized 12 hours after birth to receive total enteral nutrition (TEN) as formula; TEN + GOS (4 g/L), TEN + PDX (4 g/L), or TEN + GOS + PDX (2 g/L each) for 7 days or were supported by total parenteral nutrition (TPN) as a positive control for BT (n = 8). Blood, spleen, liver, and mesenteric lymph node (MLN) samples were cultured for aerobic and anaerobic bacteria. Colon microbiota 16S rDNA was measured by polymerase chain reaction. Myeloperoxidase activity and tumor necrosis factor-α expression were measured in ileum and ascending colon. Results: Among the enterally fed groups, no difference was seen in the Lactobacillus and Bacteroides 16S rDNA copies per gram of colonic contents, yet total bacterial levels were lower (P < 0.05) in the TEN + GOS group compared with TEN alone. Bacteria were detected in the blood, liver spleen, and MLN of TPN piglets. In contrast, bacterial counts were predominantly detected in the MLN of TEN piglets, at much lower levels than in TPN, and levels were not affected by GOS and PDX addition. TPN piglets had elevated (P < 0.05) ileal myeloperoxidase activity and a trend in elevated ascending colon tumor necrosis factor-α expression (P = 0.1). Conclusions: PDX and GOS added to formula do not induce BT in healthy piglets. Low levels of bacteria in MLN of healthy neonatal piglets may reflect mucosal sampling rather than pathological BT.

Original languageEnglish (US)
Pages (from-to)210-216
Number of pages7
JournalJournal of Pediatric Gastroenterology and Nutrition
Volume52
Issue number2
DOIs
StatePublished - Feb 1 2011
Externally publishedYes

Keywords

  • bacteria
  • prebiotic
  • translocation

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Gastroenterology

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