Acute exposure to physiological doses of triiodothyronine does not induce gonadal caspase 3 activity in goldfish in vitro

Euan R.O. Allan, Camila B. Dores, Erik R. Nelson, Hamid R. Habibi

Research output: Contribution to journalArticle

Abstract

Seasonally spawning fish rely on a dynamic and complex hormonal interplay to regulate cycles of gonadal development and the regression. Thyroid hormones have been shown to be a key player during gonadal development, and can regulate the activity of a number of essential reproductive hormones. Apoptosis is a vital cellular process that contributes to the hormonal control of gonadal development and regression, but the roles of thyroid hormones on gonadal apoptosis in goldfish have not been explored. The present study examines the role of acute T3 exposure on caspase 3-dependent apoptosis in dispersed goldfish gonadal tissue in vitro. We examined the levels of caspase 3 activity in early, mid, and late recrudescent gonadal tissue after exposure to physiological doses of T3 for up to 24 h. Acute treatment with T3 did not alter basal caspase 3 activity in goldfish gonads in vitro in these reproductive stages. This initial study suggests that transient increases in T3 levels are unlikely to directly contribute to basal caspase 3-dependent apoptosis in the gonadal tissue of goldfish, although we cannot rule out an interaction of T3 with other hormones involved in the control of apoptosis in the testis and ovary.

Original languageEnglish (US)
Article number113382
JournalGeneral and Comparative Endocrinology
Volume289
DOIs
StatePublished - Apr 1 2020

Fingerprint

Goldfish
acute exposure
goldfish
Triiodothyronine
triiodothyronine
caspase-3
Caspase 3
apoptosis
Apoptosis
dosage
thyroid hormones
Thyroid Hormones
hormones
Hormones
hormonal regulation
Gonads
Testis
gonads
Ovary
testes

Keywords

  • Caspase 3
  • Goldfish
  • Ovary
  • Testis
  • Triiodothyronine

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

Cite this

Acute exposure to physiological doses of triiodothyronine does not induce gonadal caspase 3 activity in goldfish in vitro. / Allan, Euan R.O.; Dores, Camila B.; Nelson, Erik R.; Habibi, Hamid R.

In: General and Comparative Endocrinology, Vol. 289, 113382, 01.04.2020.

Research output: Contribution to journalArticle

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