Active and inactive forms of biotin synthase occur in Heterodera glycines

Khee Man Kwon, Sadia Bekal, Leslie L. Domier, Kris N. Lambert

Research output: Contribution to journalArticle

Abstract

Heterodera glycines, the soybean cyst nematode (SCN), is a plant-parasitic nematode capable of manipulating host plant biochemistry and development. Many studies have suggested that the nematode has acquired genes from bacteria via horizontal gene transfer events (HGTs) that have the potential to enhance nematode parasitism. A recent allelic imbalance analysis identified two candidate virulence genes, which also appear to have entered the SCN genome through HGTs. One of the candidate genes, H. glycines biotin synthase (HgBioB), contained sequence polymorphisms between avirulent and virulent inbred SCN strains. To test the function of these HgBioB alleles, a complementation experiment using biotin synthase-deficient Escherichia coli was conducted. Here, we report that avirulent nematodes produce an active biotin synthase while virulent ones contain an inactive form of the enzyme. Moreover, sequencing analysis of HgBioB genes from SCN field populations indicates the presence of diverse mixture of HgBioB alleles with the virulent form being the most prevalent. We hypothesize that the mutations in the inactive HgBioB allele within the virulent SCN could result in a change in protein function that in some unknown way bolster its parasitic lifestyle.

Original languageEnglish (US)
Article numbere2019-69
JournalJournal of Nematology
Volume51
Issue number1
DOIs
StatePublished - Jan 1 2019

Keywords

  • Biochemistry
  • Biotin
  • Biotin synthase
  • Dethiobiotin
  • Heterodera glycines
  • Horizontal gene transfer
  • Soybean cyst nematode

ASJC Scopus subject areas

  • Agronomy and Crop Science

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