Activation of the pacidamycin pacl adenylation domain by MbtH-like proteins

Wenjun Zhang, John R. Heemstra, Christopher T. Walsh, Heidi J. Imker

Research output: Contribution to journalArticle

Abstract

Nonribosomal peptide synthetase (NRPS) assembly lines are major avenues for the biosynthesis of a vast array of peptidyl natural products. Several hundred bacterial NRPS gene clusters contain a small (∼70-residue) protein belonging to the MbtH family for which no function has been defined. Here we show that two strictly conserved Trp residues in MbtH-like proteins contribute to stimulation of amino acid adenylation in some NRPS modules. We also demonstrate that adenylation can be stimulated not only by cognate MbtH-like proteins but also by homologues from disparate natural product pathways.

Original languageEnglish (US)
Pages (from-to)9946-9947
Number of pages2
JournalBiochemistry
Volume49
Issue number46
DOIs
StatePublished - Nov 23 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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