@article{cc5dda0d0fdd48b6aa1c03b0f03d8d0a,
title = "Activation of silent biosynthetic gene clusters using transcription factor decoys",
abstract = "Here we report a transcription factor decoy strategy for targeted activation of eight large silent polyketide synthase and non-ribosomal peptide synthetase gene clusters, ranging from 50 to 134 kilobases (kb) in multiple streptomycetes, and characterization of a novel oxazole family compound produced by a 98-kb biosynthetic gene cluster. Owing to its simplicity and ease of use, this strategy can be scaled up readily for discovery of natural products in streptomycetes.",
author = "Bin Wang and Fang Guo and Dong, {Shi Hui} and Huimin Zhao",
note = "This work was supported by grant GM077596 (to H.Z.) from the US National Institutes of Health (NIH). Some of the data were collected in the Carl R. Woese Institute for Genomic Biology Core on a 600MHz NMR funded by NIH grant number S10-RR028833, LC-MS at the MCB Metabolomics Center, and HRMS at the SCS Mass Spectrometry Laboratory. B.W. and F.G. dedicate this article to the memory of Keqian Yang, who made important contributions to their understanding of Streptomyces genetic regulation of secondary metabolism.",
year = "2019",
month = feb,
day = "1",
doi = "10.1038/s41589-018-0187-0",
language = "English (US)",
volume = "15",
pages = "111--114",
journal = "Nature chemical biology",
issn = "1552-4450",
publisher = "Nature Research",
number = "2",
}