Activation of silent biosynthetic gene clusters using transcription factor decoys

Bin Wang, Fang Guo, Shi Hui Dong, Huimin Zhao

Research output: Contribution to journalArticle

Abstract

Here we report a transcription factor decoy strategy for targeted activation of eight large silent polyketide synthase and non-ribosomal peptide synthetase gene clusters, ranging from 50 to 134 kilobases (kb) in multiple streptomycetes, and characterization of a novel oxazole family compound produced by a 98-kb biosynthetic gene cluster. Owing to its simplicity and ease of use, this strategy can be scaled up readily for discovery of natural products in streptomycetes.

Original languageEnglish (US)
Pages (from-to)111-114
Number of pages4
JournalNature chemical biology
Volume15
Issue number2
DOIs
StatePublished - Feb 1 2019

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Multigene Family
Transcription Factors
Oxazoles
Peptide Synthases
Polyketide Synthases
Biological Products

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Activation of silent biosynthetic gene clusters using transcription factor decoys. / Wang, Bin; Guo, Fang; Dong, Shi Hui; Zhao, Huimin.

In: Nature chemical biology, Vol. 15, No. 2, 01.02.2019, p. 111-114.

Research output: Contribution to journalArticle

Wang, Bin ; Guo, Fang ; Dong, Shi Hui ; Zhao, Huimin. / Activation of silent biosynthetic gene clusters using transcription factor decoys. In: Nature chemical biology. 2019 ; Vol. 15, No. 2. pp. 111-114.
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