Activation of silent biosynthetic gene clusters using transcription factor decoys

Bin Wang, Fang Guo, Shi Hui Dong, Huimin Zhao

Research output: Contribution to journalArticlepeer-review

Abstract

Here we report a transcription factor decoy strategy for targeted activation of eight large silent polyketide synthase and non-ribosomal peptide synthetase gene clusters, ranging from 50 to 134 kilobases (kb) in multiple streptomycetes, and characterization of a novel oxazole family compound produced by a 98-kb biosynthetic gene cluster. Owing to its simplicity and ease of use, this strategy can be scaled up readily for discovery of natural products in streptomycetes.

Original languageEnglish (US)
Pages (from-to)111-114
Number of pages4
JournalNature chemical biology
Volume15
Issue number2
DOIs
StatePublished - Feb 1 2019

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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