TY - JOUR
T1 - Activation of long chain fatty acids with acyl carrier protein
T2 - demonstration of a new enzyme, acyl acyl carrier protein synthetase, in Escherichia coli
AU - Ray, T. K.
AU - Cronan, J. E.
PY - 1976
Y1 - 1976
N2 - A soluble enzyme activity which catalyzes the synthesis of acyl acyl carrier protein from acyl carrier proteins, a long chain fatty acid, and ATP has been demonstrated in E. coli. The reaction requires high concentrations of both Ca++ and Mg++ for activity, and cleaves ATP to AMP and PP(i). The fatty acyl product has been identified as acyl acyl carrier protein by its solubility, thioester linkage, molecular weight, charge, and biological activity. Several criteria indicate the enzyme is distinct from acyl CoA synthetase. The fatty acid specificity of the enzyme suggests a role of acyl acyl carrier protein synthetase in the incorporation of fatty acids into phospholipid.
AB - A soluble enzyme activity which catalyzes the synthesis of acyl acyl carrier protein from acyl carrier proteins, a long chain fatty acid, and ATP has been demonstrated in E. coli. The reaction requires high concentrations of both Ca++ and Mg++ for activity, and cleaves ATP to AMP and PP(i). The fatty acyl product has been identified as acyl acyl carrier protein by its solubility, thioester linkage, molecular weight, charge, and biological activity. Several criteria indicate the enzyme is distinct from acyl CoA synthetase. The fatty acid specificity of the enzyme suggests a role of acyl acyl carrier protein synthetase in the incorporation of fatty acids into phospholipid.
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U2 - 10.1073/pnas.73.12.4374
DO - 10.1073/pnas.73.12.4374
M3 - Article
C2 - 794875
AN - SCOPUS:0017043386
VL - 73
SP - 4374
EP - 4378
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 12
ER -