Activation of caspase-9 with irradiation inhibits invasion and angiogenesis in SNB19 human glioma cells

Niranjan Yanamandra, Shakuntala Kondraganti, Srinivasa M. Srinivasula, Meena Gujrati, William C. Olivero, Dzung H. Dinh, Jasti S. Rao

Research output: Contribution to journalArticle

Abstract

Glioblastoma multiforme, the most common brain tumor, typically exhibits markedly increased angiogenesis, which is crucial for tumor growth and invasion. Antiangiogenic strategies based on disruption of the tumor microvasculature have proven effective for the treatment of experimental brain tumors. Here, we have overexpressed human caspase-9 by stable transfection in the SNB19 glioblastoma cell line, which normally expresses low levels of caspase-9. Our studies revealed that overexpression of caspase-9 coupled with radiation has a synergistic effect on the inhibition of glioma invasion as demonstrated by Matrigel assay (>65%). Furthermore, sense caspase stable clones cocultured with fetal rat brain aggregates along with radiation showed complete inhibition as compared to the parental and vector controls. During in vitro angiogenesis, SNB19 cells cocultured with human microvascular endothelial cells (HMEC) showed vascular network formation after 48-72 h. In contrast, these capillary-like structures were inhibited when HMEC cells were cocultured with sense caspase stable SNB19 cells. This effect was further enhanced by radiation (5Gy). Signaling mechanisms revealed that apoptosis is induced by cleavage of caspase-9 by radiation, loss of mitochondrial membrane potential and activation of caspase-3. These results demonstrate that activation of caspase-9 disrupts glioma cell invasion and angiogenesis in vitro. Hence, overexpression of proapoptotic molecules such as caspase-9 may be an important determinant of the therapeutic effect of radiation in cancer therapy.

Original languageEnglish (US)
Pages (from-to)2339-2346
Number of pages8
JournalOncogene
Volume23
Issue number13
DOIs
StatePublished - Mar 25 2004

Fingerprint

Caspase 9
Glioma
Radiation
Glioblastoma
Caspases
Brain Neoplasms
Endothelial Cells
Neoplasms
Mitochondrial Membrane Potential
Therapeutic Uses
Microvessels
Caspase 3
Transfection
Clone Cells
Apoptosis
Cell Line
Brain
Growth

Keywords

  • Caspase 9
  • Glioma
  • In version angiogenesis
  • Radiation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Yanamandra, N., Kondraganti, S., Srinivasula, S. M., Gujrati, M., Olivero, W. C., Dinh, D. H., & Rao, J. S. (2004). Activation of caspase-9 with irradiation inhibits invasion and angiogenesis in SNB19 human glioma cells. Oncogene, 23(13), 2339-2346. https://doi.org/10.1038/sj.onc.1207406

Activation of caspase-9 with irradiation inhibits invasion and angiogenesis in SNB19 human glioma cells. / Yanamandra, Niranjan; Kondraganti, Shakuntala; Srinivasula, Srinivasa M.; Gujrati, Meena; Olivero, William C.; Dinh, Dzung H.; Rao, Jasti S.

In: Oncogene, Vol. 23, No. 13, 25.03.2004, p. 2339-2346.

Research output: Contribution to journalArticle

Yanamandra, N, Kondraganti, S, Srinivasula, SM, Gujrati, M, Olivero, WC, Dinh, DH & Rao, JS 2004, 'Activation of caspase-9 with irradiation inhibits invasion and angiogenesis in SNB19 human glioma cells', Oncogene, vol. 23, no. 13, pp. 2339-2346. https://doi.org/10.1038/sj.onc.1207406
Yanamandra N, Kondraganti S, Srinivasula SM, Gujrati M, Olivero WC, Dinh DH et al. Activation of caspase-9 with irradiation inhibits invasion and angiogenesis in SNB19 human glioma cells. Oncogene. 2004 Mar 25;23(13):2339-2346. https://doi.org/10.1038/sj.onc.1207406
Yanamandra, Niranjan ; Kondraganti, Shakuntala ; Srinivasula, Srinivasa M. ; Gujrati, Meena ; Olivero, William C. ; Dinh, Dzung H. ; Rao, Jasti S. / Activation of caspase-9 with irradiation inhibits invasion and angiogenesis in SNB19 human glioma cells. In: Oncogene. 2004 ; Vol. 23, No. 13. pp. 2339-2346.
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