Activating Effects of Phenolics from Apache Red Zea mays L. on Free Fatty Acid Receptor 1 and Glucokinase Evaluated with a Dual Culture System with Epithelial, Pancreatic, and Liver Cells

Diego A. Luna-Vital, Laura Chatham, John A Juvik, Vijay Singh, Pavel Somavat, Elvira Demejia

Research output: Contribution to journalArticle

Abstract

The aim was to characterize a phenolic-rich water extract from the pericarp of an improved genotype of Apache red maize (RPE) and evaluate its ability to activate the type 2 diabetes markers free fatty acid receptor 1 (GPR40) and glucokinase (GK) in vitro. The extract contained mainly phenolic acids, anthocyanins, and other flavonoids. RPE inhibited α-amylase (IC50 = 88.3 μg/mL), α-glucosidase (IC50 = 169.3 μg/mL), and reduced glucose transport in a Caco-2 cell monolayer (up to 25%). Furthermore, RPE activated GPR40 (EC50 = 77.7 μg/mL) in pancreatic INS-1E cells and GK (EC50 = 43.4 μg/mL) in liver HepG2 cells, potentially through allosteric modulation. RPE activated GPR40-related insulin secretory pathway and activated the glucose metabolism regulator AMPK (up to 78%). Our results support the hypothesis that foods with a high concentration of anthocyanins and phenolic acids, such as in the selected variety of maize used, could ameliorate obesity and type 2 diabetes comorbidities.

Original languageEnglish (US)
Pages (from-to)9148-9159
Number of pages12
JournalJournal of Agricultural and Food Chemistry
Volume67
Issue number33
DOIs
StatePublished - Aug 21 2019

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glucokinase
Glucokinase
Anthocyanins
Medical problems
phenolic acids
Nonesterified Fatty Acids
noninsulin-dependent diabetes mellitus
Liver
Type 2 Diabetes Mellitus
hepatocytes
Inhibitory Concentration 50
Zea mays
inhibitory concentration 50
free fatty acids
anthocyanins
Glucosidases
Glucose
receptors
glucose
glucosidases

Keywords

  • anthocyanins
  • colored maize
  • free fatty acid receptor 1
  • type 2 diabetes

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

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title = "Activating Effects of Phenolics from Apache Red Zea mays L. on Free Fatty Acid Receptor 1 and Glucokinase Evaluated with a Dual Culture System with Epithelial, Pancreatic, and Liver Cells",
abstract = "The aim was to characterize a phenolic-rich water extract from the pericarp of an improved genotype of Apache red maize (RPE) and evaluate its ability to activate the type 2 diabetes markers free fatty acid receptor 1 (GPR40) and glucokinase (GK) in vitro. The extract contained mainly phenolic acids, anthocyanins, and other flavonoids. RPE inhibited α-amylase (IC50 = 88.3 μg/mL), α-glucosidase (IC50 = 169.3 μg/mL), and reduced glucose transport in a Caco-2 cell monolayer (up to 25{\%}). Furthermore, RPE activated GPR40 (EC50 = 77.7 μg/mL) in pancreatic INS-1E cells and GK (EC50 = 43.4 μg/mL) in liver HepG2 cells, potentially through allosteric modulation. RPE activated GPR40-related insulin secretory pathway and activated the glucose metabolism regulator AMPK (up to 78{\%}). Our results support the hypothesis that foods with a high concentration of anthocyanins and phenolic acids, such as in the selected variety of maize used, could ameliorate obesity and type 2 diabetes comorbidities.",
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author = "Luna-Vital, {Diego A.} and Laura Chatham and Juvik, {John A} and Vijay Singh and Pavel Somavat and Elvira Demejia",
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T1 - Activating Effects of Phenolics from Apache Red Zea mays L. on Free Fatty Acid Receptor 1 and Glucokinase Evaluated with a Dual Culture System with Epithelial, Pancreatic, and Liver Cells

AU - Luna-Vital, Diego A.

AU - Chatham, Laura

AU - Juvik, John A

AU - Singh, Vijay

AU - Somavat, Pavel

AU - Demejia, Elvira

PY - 2019/8/21

Y1 - 2019/8/21

N2 - The aim was to characterize a phenolic-rich water extract from the pericarp of an improved genotype of Apache red maize (RPE) and evaluate its ability to activate the type 2 diabetes markers free fatty acid receptor 1 (GPR40) and glucokinase (GK) in vitro. The extract contained mainly phenolic acids, anthocyanins, and other flavonoids. RPE inhibited α-amylase (IC50 = 88.3 μg/mL), α-glucosidase (IC50 = 169.3 μg/mL), and reduced glucose transport in a Caco-2 cell monolayer (up to 25%). Furthermore, RPE activated GPR40 (EC50 = 77.7 μg/mL) in pancreatic INS-1E cells and GK (EC50 = 43.4 μg/mL) in liver HepG2 cells, potentially through allosteric modulation. RPE activated GPR40-related insulin secretory pathway and activated the glucose metabolism regulator AMPK (up to 78%). Our results support the hypothesis that foods with a high concentration of anthocyanins and phenolic acids, such as in the selected variety of maize used, could ameliorate obesity and type 2 diabetes comorbidities.

AB - The aim was to characterize a phenolic-rich water extract from the pericarp of an improved genotype of Apache red maize (RPE) and evaluate its ability to activate the type 2 diabetes markers free fatty acid receptor 1 (GPR40) and glucokinase (GK) in vitro. The extract contained mainly phenolic acids, anthocyanins, and other flavonoids. RPE inhibited α-amylase (IC50 = 88.3 μg/mL), α-glucosidase (IC50 = 169.3 μg/mL), and reduced glucose transport in a Caco-2 cell monolayer (up to 25%). Furthermore, RPE activated GPR40 (EC50 = 77.7 μg/mL) in pancreatic INS-1E cells and GK (EC50 = 43.4 μg/mL) in liver HepG2 cells, potentially through allosteric modulation. RPE activated GPR40-related insulin secretory pathway and activated the glucose metabolism regulator AMPK (up to 78%). Our results support the hypothesis that foods with a high concentration of anthocyanins and phenolic acids, such as in the selected variety of maize used, could ameliorate obesity and type 2 diabetes comorbidities.

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