Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells

Eddie Y. Chung, Christopher J. Ochs, Yi Wang, Lei Lei, Qin Qin, Andrew M. Smith, Alex Y. Strongin, Roger Kamm, Ying Xin Qi, Shaoying Lu, Yingxiao Wang

Research output: Contribution to journalArticle

Abstract

We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells.

Original languageEnglish (US)
Pages (from-to)5025-5032
Number of pages8
JournalNano letters
Volume15
Issue number8
DOIs
StatePublished - Aug 12 2015

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Keywords

  • FRET nanosensor
  • MT1-MMP
  • activatable cell-penetrating peptide
  • cancer
  • multiplex signals
  • single cell

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Chung, E. Y., Ochs, C. J., Wang, Y., Lei, L., Qin, Q., Smith, A. M., Strongin, A. Y., Kamm, R., Qi, Y. X., Lu, S., & Wang, Y. (2015). Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells. Nano letters, 15(8), 5025-5032. https://doi.org/10.1021/acs.nanolett.5b01047