Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells

Eddie Y. Chung; Christopher J. Ochs; Yi Wang; Lei Lei; Qin Qin; Andrew M. Smith; Alex Y. Strongin; Roger Kamm; Ying Xin Qi; Shaoying Lu; Yingxiao Wang

doi:10.1021/acs.nanolett.5b01047

Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells

Eddie Y. Chung, Christopher J. Ochs, Yi Wang, Lei Lei, Qin Qin, Andrew M. Smith, Alex Y. Strongin, Roger Kamm, Ying Xin Qi, Shaoying Lu, Yingxiao Wang

Research output: Contribution to journal › Article › peer-review

Abstract

We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells.

Original language	English (US)
Pages (from-to)	5025-5032
Number of pages	8
Journal	Nano letters
Volume	15
Issue number	8
DOIs	https://doi.org/10.1021/acs.nanolett.5b01047
State	Published - Aug 12 2015

Keywords

FRET nanosensor
MT1-MMP
activatable cell-penetrating peptide
cancer
multiplex signals
single cell

ASJC Scopus subject areas

Bioengineering
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
Mechanical Engineering

Online availability

10.1021/acs.nanolett.5b01047

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title = "Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells",

abstract = "We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells.",

keywords = "FRET nanosensor, MT1-MMP, activatable cell-penetrating peptide, cancer, multiplex signals, single cell",

author = "Chung, {Eddie Y.} and Ochs, {Christopher J.} and Yi Wang and Lei Lei and Qin Qin and Smith, {Andrew M.} and Strongin, {Alex Y.} and Roger Kamm and Qi, {Ying Xin} and Shaoying Lu and Yingxiao Wang",

note = "Publisher Copyright: {\textcopyright} 2015 American Chemical Society.",

year = "2015",

month = aug,

day = "12",

doi = "10.1021/acs.nanolett.5b01047",

language = "English (US)",

volume = "15",

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journal = "Nano Letters",

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AU - Chung, Eddie Y.

AU - Ochs, Christopher J.

AU - Wang, Yi

AU - Lei, Lei

AU - Qin, Qin

AU - Smith, Andrew M.

AU - Strongin, Alex Y.

AU - Kamm, Roger

AU - Qi, Ying Xin

AU - Lu, Shaoying

AU - Wang, Yingxiao

PY - 2015/8/12

Y1 - 2015/8/12

N2 - We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells.

AB - We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells.

KW - FRET nanosensor

KW - MT1-MMP

KW - activatable cell-penetrating peptide

KW - cancer

KW - multiplex signals

KW - single cell

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Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells

Abstract

Keywords

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