Action of the thiamine protagonist bacimethrin on thiamine biosynthesis

J. L. Zilles, L. R. Croal, D. M. Downs

Research output: Contribution to journalArticlepeer-review


Bacimethrin is an analog of the 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine and inhibits the growth of Salmonella enterica serovar Typhimurium on a defined medium. Two classes of mutants that had increased bacimethrin resistance were isolated and characterized. Results showed that over-expression of the thi operon or specific lesions in thiD resulted in a bacimethrin-resistant phenotype. Phenotypic analyses of the thiD mutants suggested that they had a specific defect in one of the two kinase activities associated with this gene product and, further, that ThiD and not PdxK was primarily responsible for salvage of HMP from the medium.

Original languageEnglish (US)
Pages (from-to)5606-5610
Number of pages5
JournalJournal of bacteriology
Issue number19
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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