Actin protrusions push at apical junctions to maintain E-cadherin adhesion

John Xiao He Li, Vivian W. Tang, William M. Brieher

Research output: Contribution to journalArticle

Abstract

Cadherin-mediated cell–cell adhesion is actin-dependent, but the precise role of actin in maintaining cell–cell adhesion is not fully understood. Actin polymerization-dependent protrusive activity is required to push distally separated cells close enough to initiate contact. Whether protrusive activity is required to maintain adhesion in confluent sheets of epithelial cells is not known. By electron microscopy as well as live cell imaging, we have identified a population of protruding actin microspikes that operate continuously near apical junctions of polarized Madin-Darby canine kidney (MDCK) cells. Live imaging shows that microspikes containing E-cadherin extend into gaps between E-cadherin clusters on neighboring cells, while reformation of cadherin clusters across the cell–cell boundary correlates with microspike withdrawal. We identify Arp2/3, EVL, and CRMP-1 as 3 actin assembly factors necessary for microspike formation. Depleting these factors from cells using RNA interference (RNAi) results in myosin II-dependent unzipping of cadherin adhesive bonds. Therefore, actin polymerization-dependent protrusive activity operates continuously at cadherin cell–cell junctions to keep them shut and to prevent myosin II-dependent contractility from tearing cadherin adhesive contacts apart.

Original languageEnglish (US)
Pages (from-to)432-438
Number of pages7
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number1
DOIs
StatePublished - Jan 7 2020

Fingerprint

Cadherins
Actins
Myosin Type II
Polymerization
Adhesives
Madin Darby Canine Kidney Cells
RNA Interference
Electron Microscopy
Epithelial Cells
Population

Keywords

  • Actin
  • Adhesion
  • E-cadherin
  • Epithelial
  • Junction

ASJC Scopus subject areas

  • General

Cite this

Actin protrusions push at apical junctions to maintain E-cadherin adhesion. / He Li, John Xiao; Tang, Vivian W.; Brieher, William M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 117, No. 1, 07.01.2020, p. 432-438.

Research output: Contribution to journalArticle

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