Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus

Satoru Konnai, Saiki Imamura, Chie Nakajima, William Harold Witola, Shinji Yamada, Martin Simuunza, Andrew Nambota, Jun Yasuda, Kazuhiko Ohashi, Misao Onuma

Research output: Research - peer-reviewArticle

Abstract

In order to investigate the transmission dynamics of Theileria parva (T. parva) by the brown ear tick, Rhipicephalus appendiculatus (R. appendiculatus), under experimental conditions, detection of T. parva in ticks and cattle was performed by a quantitative real-time PCR assay. A calf inoculated with a T. parva mixture became PCR-positive for T. parva infection on day 8 post-inoculation, and subsequently, nymphal ticks were introduced and maintained to feed on the infected calf for 6 days. Engorged nymphs were collected daily and allowed to molt into adults, and overall, 70.8% (121/171) of the adult ticks acquired the T. parva infection. Furthermore, the T. parva infection rate in ticks under field conditions was monitored by real-time PCR in R. appendiculatus ticks collected from a traditionally managed pastoral land of Zambia, on which Sanga breed cattle are traditionally reared and the area has endemic East Coast fever (ECF). A total of 70 cattle were randomly selected in the same area and 67 (95.7%) were found to be serologically positive for R. appendiculatus tick antigen (RIM36). Twenty-nine (43.3%) of the 67 serologically positive cattle were real-time PCR-positive for T. parva, although no piroplasms could be detected in the blood smears. Unexpectedly, out of 614 R. appendiculatus nymphal and adult ticks collected by flagging vegetation, 4.1% were positive for T. parva DNA. However, since the rate of transmission of T. parva from infected cattle to ticks and vice versa and the serological evidence of exposure to R. appendiculatus ticks in naturally exposed cattle were relatively high, it would be wise in such a case to consider vector control as well as vaccination against ECF as control measures.

LanguageEnglish (US)
Pages34-41
Number of pages8
JournalActa Tropica
Volume99
Issue number1
DOIs
StatePublished - Aug 2006
Externally publishedYes

Fingerprint

Theileria parva
Rhipicephalus
Ticks
Real-Time Polymerase Chain Reaction
Infection
Theileriasis
Zambia
Nymph
Ear
Vaccination
Antigens
Polymerase Chain Reaction
DNA

Keywords

  • Detection
  • Monitoring
  • Real-time PCR
  • Theileria parva
  • Transmission

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Konnai, S., Imamura, S., Nakajima, C., Witola, W. H., Yamada, S., Simuunza, M., ... Onuma, M. (2006). Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus. Acta Tropica, 99(1), 34-41. DOI: 10.1016/j.actatropica.2006.06.008

Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus. / Konnai, Satoru; Imamura, Saiki; Nakajima, Chie; Witola, William Harold; Yamada, Shinji; Simuunza, Martin; Nambota, Andrew; Yasuda, Jun; Ohashi, Kazuhiko; Onuma, Misao.

In: Acta Tropica, Vol. 99, No. 1, 08.2006, p. 34-41.

Research output: Research - peer-reviewArticle

Konnai, S, Imamura, S, Nakajima, C, Witola, WH, Yamada, S, Simuunza, M, Nambota, A, Yasuda, J, Ohashi, K & Onuma, M 2006, 'Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus' Acta Tropica, vol 99, no. 1, pp. 34-41. DOI: 10.1016/j.actatropica.2006.06.008
Konnai S, Imamura S, Nakajima C, Witola WH, Yamada S, Simuunza M et al. Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus. Acta Tropica. 2006 Aug;99(1):34-41. Available from, DOI: 10.1016/j.actatropica.2006.06.008
Konnai, Satoru ; Imamura, Saiki ; Nakajima, Chie ; Witola, William Harold ; Yamada, Shinji ; Simuunza, Martin ; Nambota, Andrew ; Yasuda, Jun ; Ohashi, Kazuhiko ; Onuma, Misao. / Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus. In: Acta Tropica. 2006 ; Vol. 99, No. 1. pp. 34-41
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