TY - JOUR
T1 - Acquired thermotolerance and heat shock proteins in thermophiles from the three phylogenetic domains
AU - Trent, J. D.
AU - Gabrielsen, M.
AU - Jensen, B.
AU - Neuhard, J.
AU - Olsen, J.
PY - 1994
Y1 - 1994
N2 - Thermophilic organisms from each of the three phylogenetic domains (Bacteria, Archaea, and Eucarya) acquired thermotolerance after heat shock. Bacillus caldolyticus grown at 60°C and heat shocked at 69°C for 10 min showed thermotolerance at 74°C, Sulfolobus shibatae grown at 70°C and heat shocked at 88°C for 60 min showed thermotolerance at 95°C, and Thermomyces lanuginosus grown at 50°C and heat shocked at 55°C for 60 min showed thermotolerance at 58°C. Determinations of protein synthesis during heat shock revealed differences in the dominant heat shock proteins for each species. For B. caldolyticus, a 70-kDa protein dominated while for S. shibatae, a 55-kDa protein dominated and for T. lanuginosus, 31- to 33-kDa proteins dominated. Reagents that disrupted normal protein synthesis during heat shock prevented the enhanced thermotolerance.
AB - Thermophilic organisms from each of the three phylogenetic domains (Bacteria, Archaea, and Eucarya) acquired thermotolerance after heat shock. Bacillus caldolyticus grown at 60°C and heat shocked at 69°C for 10 min showed thermotolerance at 74°C, Sulfolobus shibatae grown at 70°C and heat shocked at 88°C for 60 min showed thermotolerance at 95°C, and Thermomyces lanuginosus grown at 50°C and heat shocked at 55°C for 60 min showed thermotolerance at 58°C. Determinations of protein synthesis during heat shock revealed differences in the dominant heat shock proteins for each species. For B. caldolyticus, a 70-kDa protein dominated while for S. shibatae, a 55-kDa protein dominated and for T. lanuginosus, 31- to 33-kDa proteins dominated. Reagents that disrupted normal protein synthesis during heat shock prevented the enhanced thermotolerance.
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U2 - 10.1128/jb.176.19.6148-6152.1994
DO - 10.1128/jb.176.19.6148-6152.1994
M3 - Comment/debate
C2 - 7928981
AN - SCOPUS:0027939923
SN - 0021-9193
VL - 176
SP - 6148
EP - 6152
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 19
ER -