Acquired thermotolerance and heat shock proteins in thermophiles from the three phylogenetic domains

J. D. Trent, M. Gabrielsen, B. Jensen, J. Neuhard, J. Olsen

Research output: Contribution to journalComment/debatepeer-review

Abstract

Thermophilic organisms from each of the three phylogenetic domains (Bacteria, Archaea, and Eucarya) acquired thermotolerance after heat shock. Bacillus caldolyticus grown at 60°C and heat shocked at 69°C for 10 min showed thermotolerance at 74°C, Sulfolobus shibatae grown at 70°C and heat shocked at 88°C for 60 min showed thermotolerance at 95°C, and Thermomyces lanuginosus grown at 50°C and heat shocked at 55°C for 60 min showed thermotolerance at 58°C. Determinations of protein synthesis during heat shock revealed differences in the dominant heat shock proteins for each species. For B. caldolyticus, a 70-kDa protein dominated while for S. shibatae, a 55-kDa protein dominated and for T. lanuginosus, 31- to 33-kDa proteins dominated. Reagents that disrupted normal protein synthesis during heat shock prevented the enhanced thermotolerance.

Original languageEnglish (US)
Pages (from-to)6148-6152
Number of pages5
JournalJournal of bacteriology
Volume176
Issue number19
DOIs
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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