Acoustic Microscopy: A Study of Contrast in Fresh Tissue

Gail Scherba; Patricia A. Hoagland; William D. O'Brien

doi:10.1109/58.294104

Acoustic Microscopy: A Study of Contrast in Fresh Tissue

Gail Scherba, Patricia A. Hoagland, William D. O'Brien

Carle Illinois College of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The scanning laser acoustic microscope (SLAM), which exhibits a resolution of about 15 µm in biological materials, was operated at a frequency of 100 MHz to evaluate the source of image contrast. Specifically, the SLAM's quantitative capabilities yielded the ultrasonic propagation properties of attenuation coefficient and propagation speed of rat brain tissue (white versus gray matter) and these properties were correlated with tissue constituents and image contrast. The SLAM's image contrast between the two brain layers in both fresh and fixed specimens was analyzed subjectively by an experienced microscopist. It was determined that ethanol fixation decreased the image contrast between the brain layers. Additionally, the propagation speed was the least affected property in the fresh tissue specimens yet increased in both brain layers after fixation whereas the attenuation coefficient of white matter in unfixed brain tissue was higher than that of gray matter. These results indicate that the SLAM's acoustic image contrast is a direct reflection of the difference in attenuation coefficient whereas the propagation speed is not a significant contributor to the image contrast.

Original language	English (US)
Pages (from-to)	451-457
Number of pages	7
Journal	IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Volume	41
Issue number	4
DOIs	https://doi.org/10.1109/58.294104
State	Published - Jul 1994

ASJC Scopus subject areas

Instrumentation
Acoustics and Ultrasonics
Electrical and Electronic Engineering

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title = "Acoustic Microscopy: A Study of Contrast in Fresh Tissue",

abstract = "The scanning laser acoustic microscope (SLAM), which exhibits a resolution of about 15 µm in biological materials, was operated at a frequency of 100 MHz to evaluate the source of image contrast. Specifically, the SLAM's quantitative capabilities yielded the ultrasonic propagation properties of attenuation coefficient and propagation speed of rat brain tissue (white versus gray matter) and these properties were correlated with tissue constituents and image contrast. The SLAM's image contrast between the two brain layers in both fresh and fixed specimens was analyzed subjectively by an experienced microscopist. It was determined that ethanol fixation decreased the image contrast between the brain layers. Additionally, the propagation speed was the least affected property in the fresh tissue specimens yet increased in both brain layers after fixation whereas the attenuation coefficient of white matter in unfixed brain tissue was higher than that of gray matter. These results indicate that the SLAM's acoustic image contrast is a direct reflection of the difference in attenuation coefficient whereas the propagation speed is not a significant contributor to the image contrast.",

author = "Gail Scherba and Hoagland, {Patricia A.} and O'Brien, {William D.}",

note = "Funding Information: Manuscript received September 14, 1993; revised january 27, 1994; accepted January 31, 1994. This work was supported in part by the National Institutes of Health and National Cancer Institute by Grants CA09067 and CA39704 G. A. Scherba is with the Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801 USA. P. A. Hoagland was with the Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, IL. She is now with Amber Leaf Veterinary Clinic, Windfield, IL 60190 USA. W. D. O{\textquoteright}Brien, Jr. is with the Department of Electrical and Computer Engineering, University of Illinois, Urbana, IL 61801 USA. IEEE Log Number 9401978.",

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N2 - The scanning laser acoustic microscope (SLAM), which exhibits a resolution of about 15 µm in biological materials, was operated at a frequency of 100 MHz to evaluate the source of image contrast. Specifically, the SLAM's quantitative capabilities yielded the ultrasonic propagation properties of attenuation coefficient and propagation speed of rat brain tissue (white versus gray matter) and these properties were correlated with tissue constituents and image contrast. The SLAM's image contrast between the two brain layers in both fresh and fixed specimens was analyzed subjectively by an experienced microscopist. It was determined that ethanol fixation decreased the image contrast between the brain layers. Additionally, the propagation speed was the least affected property in the fresh tissue specimens yet increased in both brain layers after fixation whereas the attenuation coefficient of white matter in unfixed brain tissue was higher than that of gray matter. These results indicate that the SLAM's acoustic image contrast is a direct reflection of the difference in attenuation coefficient whereas the propagation speed is not a significant contributor to the image contrast.

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Acoustic Microscopy: A Study of Contrast in Fresh Tissue

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